Anti-nmt55 / p54nrb antibody (ab70335) is a rabbit polyclonal antibody detecting nmt55 / p54nrb in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 20 publications
- Trusted since 2008
View Alternative Names
NRB54, NONO, Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, p54(nrb), p54nrb, NMT55
- WB
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Western blot - Anti-nmt55 / p54nrb antibody (AB70335)
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody (ab70335) at 0.04 µg/mL
Lane 1:
Whole cell lysate from HeLa cells at 50 µg
Lane 2:
Whole cell lysate from HeLa cells at 15 µg
Lane 3:
Whole cell lysate from HeLa cells at 5 µg
Lane 4:
Whole cell lysate from 293T cells at 50 µg
Predicted band size: 54 kDa
Observed band size: 60 kDa
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- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-nmt55 / p54nrb antibody (AB70335)
ICC/IF image of ab70335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- IHC-P
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody (AB70335)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling nmt55 / p54nrb with ab70335 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection : DAB.
- IHC-P
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody (AB70335)
IHC image of ab70335 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70335, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- IP
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Immunoprecipitation - Anti-nmt55 / p54nrb antibody (AB70335)
Detection of Human nmt55 / p54nrb by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70335 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
All lanes:
Immunoprecipitation - Anti-nmt55 / p54nrb antibody (ab70335)
Predicted band size: 54 kDa
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- WB
Supplier Data
Western blot - Anti-nmt55 / p54nrb antibody (AB70335)
All lanes:
Western blot - Anti-nmt55 / p54nrb antibody (ab70335) at 0.1 µg/mL
Lane 1:
NIH3T3 whole cell lysate at 50 µg
Lane 2:
TCMK-1 whole cell lysate at 50 µg
Lane 3:
4T1 whole cell lysate at 50 µg
Lane 4:
CT26.WT whole cell lysate at 50 µg
Lane 5:
C6 whole cell lysate at 50 µg
Predicted band size: 54 kDa
true
Exposure time: 30s
- WB
CiteAb
Western blot - Anti-nmt55 / p54nrb antibody (AB70335)
nmt55 / p54nrb western blot using anti-nmt55 / p54nrb antibody ab70335. Publication image and figure legend from Innes, B. T., Bailey, M. L., et al., 2013, Front Physiol, PubMed 23407864.
ab70335 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70335 please see the product overview.
GST pull-downs with Pin1 mutants reveal two different types of binding proteins. (A) Large-scale GST pull-downs. One to two milligrams of nocodazole-treated HeLa lysates were incubated with GST fusion proteins bound to glutathione beads. Interacting proteins were run on a 5–12% gradient gel and stained with Coomassie Blue. (B) GST pull-downs were performed as in (A) with 1 mg of HeLa lysate. Interacting proteins were run on 10% SDS-PAGE, transferred to PVDF and blotted with the indicated antibody. To better compare the amount of fusion protein on the beads, 1/200 of each pull-down was run on a gel and stained with Coomassie Blue (PD). (C) Quantification of blots shown in (B). Results are the mean of three independent pull-down experiments ±S.E.M.
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Reactivity data
Product details
Anti-nmt55 / p54nrb antibody (ab70335) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of nmt55 / p54nrb?
Anti-nmt55 / p54nrb (ab70335) specifically detects a band for nmt55 / p54nrb (UniProt: Q15233) at a molecular weight of 54kDa.
Trusted by the scientific community
Anti-nmt55 / p54nrb (ab70335) was first used in a scientific publication in 2008 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-nmt55 / p54nrb (ab70335) has over 5 independent reviews from customers.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P54nrb is a multifunctional protein critical in nuclear processes. It forms part of a complex involved in RNA processing and nuclear retention of edited RNA. This complex also includes the proteins PSF and PSPC1 contributing to its role in transcription regulation and RNA stability. p54nrb impacts gene expression through its ability to bind to DNA and RNA influencing numerous regulatory pathways within the cell.
Pathways
P54nrb has essential roles in gene expression and RNA maturation pathways. It closely interacts with the RNA polymerase II machinery affecting transcription elongation. The protein has an established connection with the steroid receptor RNA activator (SRA) to modulate transcriptional responses. It is related to other DBHS proteins such as PSF which work together for coordinate regulation of gene expression and splice site selection.
Product protocols
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Target data
Publications (33)
Recent publications for all applications. Explore the full list and refine your search
Cell death discovery 11:234 PubMed40360465
2025
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Journal of experimental & clinical cancer research : CR 44:90 PubMed40059196
2025
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Journal of experimental & clinical cancer research : CR 44:81 PubMed40033337
2025
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Genes to cells : devoted to molecular & cellular mechanisms 29:746-756 PubMed38964745
2024
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Nature structural & molecular biology 31:1341-1354 PubMed38658621
2024
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iScience 27:109278 PubMed38482502
2024
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Science advances 9:eadi4565 PubMed37851801
2023
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22862 PubMed36906291
2023
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Theranostics 12:5451-5469 PubMed35910786
2022
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Cell death & disease 13:590 PubMed35803902
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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