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AB229782

Anti-NMU antibody

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(1 Publication)

Rabbit Polyclonal NMU antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NMU.

View Alternative Names

Neuromedin-U, NMU

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NMU antibody (AB229782)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NMU antibody (AB229782)

A431 (Human epidermoid carcinoma cell line) cells stained for NMU (green) using ab229782 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue).

Western blot - Anti-NMU antibody (AB229782)
  • WB

Supplier Data

Western blot - Anti-NMU antibody (AB229782)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-NMU antibody (ab229782) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Predicted band size: 20 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NMU. The exact immunogen used to generate this antibody is proprietary information.

P48645

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuromedin U (NMU) is a regulatory peptide known for its role in modulating smooth muscle contraction and energy homeostasis. Alternate names include neuromedin reflecting its original discovery in the spinal cord. NMU has a molecular mass ranging between 2392 and 2583 Da depending on the variant. It is expressed predominantly in the gastrointestinal tract and central nervous system influencing various physiological processes within these tissues.
Biological function summary

NMU functions significantly within the nervous and endocrine systems. It is an important part of the larger neuromedin peptide family. NMU interacts with specific receptors NMU-R1 and NMU-R2 to mediate biological effects. Within the body NMU influences feeding behavior circadian rhythms and stress response mechanisms demonstrating its integral role in maintaining physiological balance.

Pathways

NMU integrates into hormonal and neuroendocrine signaling routes. The peptide participates in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis influencing stress responses. Its action also intersects the energy metabolism pathway interacting with proteins such as ghrelin and leptin. These interactions position NMU as an important player in energy intake control and stress regulation.

NMU has implications in obesity and inflammatory diseases. Abnormally high or low levels can contribute to metabolic disorders indicating its central role in energy balance and metabolic health. NMU's connection to the HPA axis associates it with proteins like corticotropin-releasing hormone which impacts inflammatory processes. Exploring NMU's involvement could unveil new therapeutic strategies for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuromedin-U-25. Ligand for receptors NMUR1 and NMUR2 (By similarity). Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.. Neuromedin precursor-related peptide 33. Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release although its potency is much lower than that of neuromedin precursor-related peptide 36.. Neuromedin precursor-related peptide 36. Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release from lactotroph cells in the pituitary gland, probably via the hypothalamic dopaminergic system.
See full target information NMU
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 9:e11512 PubMed34141479

2021

Neuromedin U and neurotensin may promote the development of the tumour microenvironment in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daheng Yang,Xianwei Zhang,Zheqian Li,Fei Xu,Chenjie Tang,Hongbing Chen
View all publications
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