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Mouse Monoclonal SCN5A antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCN5A.


Images

Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (AB62388), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (AB62388), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human SCN5A. The exact immunogen used to generate this antibody is proprietary information. Database link Q14524

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

Mouse IgM [B11/7] - Isotype control ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:1309946, PubMed:21447824, PubMed:23085483, PubMed:23420830, PubMed:25370050, PubMed:26279430, PubMed:26392562, PubMed:26776555). Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat (PubMed:11234013, PubMed:11804990, PubMed:12569159, PubMed:1309946). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (By similarity).

Alternative names

Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, hH1, SCN5A

Recommended products

Mouse Monoclonal SCN5A antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCN5A.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human SCN5A. The exact immunogen used to generate this antibody is proprietary information. Database link Q14524
Clone number
4G8:1G7
Purification technique
Affinity purification Immunogen
Specificity

The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein nNav1.5 also known as SCN5A functions as a voltage-gated sodium channel often referred to as Naᵥ1.5. It allows the rapid influx of sodium ions into cells aiding in the generation and propagation of action potentials especially within cardiac myocytes. The protein has a molecular mass of approximately 260 kDa. This channel finds high expression in cardiac tissues contributing significantly to the electrical activity of the heart muscle. While nNav1.5 is mostly found in the heart its expression can be detected albeit less prominently in other tissues like the gastrointestinal tract.

Biological function summary

NNav1.5 plays an important role in maintaining normal cardiac rhythm. It is part of a complex that includes various auxiliary proteins such as the beta subunits which modulate its activity and trafficking. nNav1.5's main function involves the initiation and conduction of electrical signals in cardiac cells synchronizing heartbeats. Besides its central role in excitability nNav1.5 may engage in other cellular processes such as mechanoelectric feedback and response to oxidative stress.

Pathways

NNav1.5 is an essential player in cardiac conduction and excitation-contraction coupling pathways. Within these pathways it interacts closely with proteins like calcium channels and potassium channels which regulate action potentials' phases and heart muscle contraction. These pathways show the intricate balance of ion exchanges responsible for cardiac rhythm and function highlighting nNav1.5's influence in the orderly contraction of the heart.

Associated diseases and disorders

Mutations or dysfunctions in nNav1.5 can lead to cardiac arrhythmias and dilated cardiomyopathy. In conditions like Long QT Syndrome nNav1.5 mutations can cause delayed repolarization of the cardiac cells leading to irregular heartbeats. Another related disorder is Brugada syndrome where nNav1.5 malfunction leads to erratic electrical activity and a risk of sudden cardiac death. The interaction of nNav1.5 with other proteins like connexins affects these diseases as connexins influence gap junction communication essential for synchronized cardiac activity.

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2 product images

  • Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388), expandable thumbnail

    Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)

    Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879>) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (Mouse IgM [B11/7] - Isotype control ab91545, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)

    ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.



    A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.



    B: DAP1 co-staining of cell nuclei.

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Product protocols

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