JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB62388

Anti-nNav1.5 antibody [4G8:1G7]

3

(3 Reviews)

|

(4 Publications)

Mouse Monoclonal SCN5A antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCN5A.

View Alternative Names

Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, hH1, SCN5A

2 Images
Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (AB62388)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (AB62388)

Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (AB62388)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (AB62388)

ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.

A : Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.

B : DAP1 co-staining of cell nuclei.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G8:1G7

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human SCN5A. The exact immunogen used to generate this antibody is proprietary information.

Q14524

Specificity

The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.

style
left-column

Complementary Products

Primary Antibodies

AB109304

Anti-BAP31 antibody [EPR3878(2)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

  • 5
  • 1
Sample Prep & Detection Kits

AB102778

Antibody Concentration And Clean-Up Kit

Other - Antibody Concentration And Clean-Up Kit (AB102778)

  • 0
  • 0
Primary Antibodies

AB181052

Anti-CaMKII delta antibody [EPR13095]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (AB181052)

  • 5
  • 1
Primary Antibodies

AB2819

Anti-SERCA1 ATPase antibody [VE121G9]

BOND RX™ Validated|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-SERCA1 ATPase antibody [VE121G9] (AB2819)

  • 4
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein nNav1.5 also known as SCN5A functions as a voltage-gated sodium channel often referred to as Naᵥ1.5. It allows the rapid influx of sodium ions into cells aiding in the generation and propagation of action potentials especially within cardiac myocytes. The protein has a molecular mass of approximately 260 kDa. This channel finds high expression in cardiac tissues contributing significantly to the electrical activity of the heart muscle. While nNav1.5 is mostly found in the heart its expression can be detected albeit less prominently in other tissues like the gastrointestinal tract.
Biological function summary

NNav1.5 plays an important role in maintaining normal cardiac rhythm. It is part of a complex that includes various auxiliary proteins such as the beta subunits which modulate its activity and trafficking. nNav1.5's main function involves the initiation and conduction of electrical signals in cardiac cells synchronizing heartbeats. Besides its central role in excitability nNav1.5 may engage in other cellular processes such as mechanoelectric feedback and response to oxidative stress.

Pathways

NNav1.5 is an essential player in cardiac conduction and excitation-contraction coupling pathways. Within these pathways it interacts closely with proteins like calcium channels and potassium channels which regulate action potentials' phases and heart muscle contraction. These pathways show the intricate balance of ion exchanges responsible for cardiac rhythm and function highlighting nNav1.5's influence in the orderly contraction of the heart.

Mutations or dysfunctions in nNav1.5 can lead to cardiac arrhythmias and dilated cardiomyopathy. In conditions like Long QT Syndrome nNav1.5 mutations can cause delayed repolarization of the cardiac cells leading to irregular heartbeats. Another related disorder is Brugada syndrome where nNav1.5 malfunction leads to erratic electrical activity and a risk of sudden cardiac death. The interaction of nNav1.5 with other proteins like connexins affects these diseases as connexins influence gap junction communication essential for synchronized cardiac activity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 1309946, PubMed : 21447824, PubMed : 23085483, PubMed : 23420830, PubMed : 25370050, PubMed : 26279430, PubMed : 26392562, PubMed : 26776555). Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat (PubMed : 11234013, PubMed : 11804990, PubMed : 12569159, PubMed : 1309946). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (By similarity).
See full target information SCN5A
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 8:903 PubMed29184507

2017

Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel G Utrilla,Paloma Nieto-Marín,Silvia Alfayate,David Tinaquero,Marcos Matamoros,Marta Pérez-Hernández,Sandra Sacristán,Lorena Ondo,Raquel de Andrés,F Javier Díez-Guerra,Juan Tamargo,Eva Delpón,Ricardo Caballero

Nature medicine 23:361-367 PubMed28191886

2017

Sirtuin 1 regulates cardiac electrical activity by deacetylating the cardiac sodium channel.

Applications

Unspecified application

Species

Unspecified reactive species

Ajit Vikram,Christopher M Lewarchik,Jin-Young Yoon,Asma Naqvi,Santosh Kumar,Gina M Morgan,Julia S Jacobs,Qiuxia Li,Young-Rae Kim,Modar Kassan,Jing Liu,Mohanad Gabani,Ajay Kumar,Haider Mehdi,Xiaodong Zhu,Xiaoqun Guan,William Kutschke,Xiaoming Zhang,Ryan L Boudreau,Shengchuan Dai,Daniel S Matasic,Saet-Byel Jung,Kenneth B Margulies,Vikas Kumar,Markus M Bachschmid,Barry London,Kaikobad Irani

International journal of molecular medicine 37:727-33 PubMed26820605

2016

A novel mutation in the SCN5A gene contributes to arrhythmogenic characteristics of early repolarization syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Guo,Lan Ren,Xuhua Chen,Cuihong Hou,Jianmin Chu,Jielin Pu,Shu Zhang

Toxicological sciences : an official journal of th 123:281-9 PubMed21693436

2011

Estimating the risk of drug-induced proarrhythmia using human induced pluripotent stem cell-derived cardiomyocytes.

Applications

ICC/IF

Species

Human

Liang Guo,Rory M C Abrams,Joshua E Babiarz,Jennifer D Cohen,Sei Kameoka,Martin J Sanders,Eric Chiao,Kyle L Kolaja
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com