Mouse Recombinant Monoclonal nNOS antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended |
Rat | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1
Mouse Recombinant Monoclonal nNOS antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
16/nNOS/NOS Type I
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.
NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.
NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney (PMID:10620216).
The expression profile observed is consistent with what has been described in the literature (PMID: 15486091).
The identity of the bands between 50 kDa and 75 kDa are unknown.
Exposure time: 59 seconds.
All lanes: Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate 20 μg
Lane 2: Mouse cerebellum tissue lysate 20 μg
Lane 3: Mouse hippocampus tissue lysate 20 μg
Lane 4: Mouse kidney tissue lysate 20 μg
Lane 5: Rat brain tissue lysate 20 μg
Lane 6: Rat cerebellum tissue lysate 20 μg
Lane 7: Rat hippocampus tissue lysate 20 μg
Lane 8: Rat kidney tissue lysate 20 μg
Observed band size: 125 kDa, 160 kDa
Exposure time: 59s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney (PMID:10620216).
The expression profile observed is consistent with what has been described in the literature (PMID: 15486091).
The identity of the bands between 50 kDa and 75 kDa are unknown.
Exposure time: 180 seconds.
All lanes: Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate 20 μg
Lane 2: Human kidney tissue lysate 20 μg
Observed band size: 125 kDa, 160 kDa
Exposure time: 180s
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