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Mouse Recombinant Monoclonal nNOS antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (AB307562), expandable thumbnail
  • Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (AB307562), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Alternative names

Recommended products

Mouse Recombinant Monoclonal nNOS antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

16/nNOS/NOS Type I

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.

Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

Associated diseases and disorders

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562), expandable thumbnail

    Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: kidney (PMID:10620216).

    The expression profile observed is consistent with what has been described in the literature (PMID: 15486091).

    The identity of the bands between 50 kDa and 75 kDa are unknown.

    Exposure time: 59 seconds.

    All lanes: Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate 20 μg

    Lane 2: Mouse cerebellum tissue lysate 20 μg

    Lane 3: Mouse hippocampus tissue lysate 20 μg

    Lane 4: Mouse kidney tissue lysate 20 μg

    Lane 5: Rat brain tissue lysate 20 μg

    Lane 6: Rat cerebellum tissue lysate 20 μg

    Lane 7: Rat hippocampus tissue lysate 20 μg

    Lane 8: Rat kidney tissue lysate 20 μg

    Observed band size: 125 kDa, 160 kDa

    Exposure time: 59s

  • Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562), expandable thumbnail

    Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: kidney (PMID:10620216).

    The expression profile observed is consistent with what has been described in the literature (PMID: 15486091).

    The identity of the bands between 50 kDa and 75 kDa are unknown.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-nNOS (neuronal) antibody [16/nNOS/NOS Type I] (ab307562) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate 20 μg

    Lane 2: Human kidney tissue lysate 20 μg

    Observed band size: 125 kDa, 160 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com