AB1376

Anti-nNOS (neuronal) antibody

Name: Anti-nNOS (neuronal) antibody (ab1376) | Abcam
Brand: Abcam Limited
SKU: AB1376
Price: 450 USD
Availability: InStock
Rating: 4 (21 reviews)

(21 Reviews)

(98 Publications)

Anti-nNOS (neuronal) antibody (ab1376) is a goat polyclonal antibody detecting nNOS (neuronal) in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Over 80 publications
- Trusted since 2002

View Alternative Names

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (AB1376)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (AB1376)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (AB1376)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (AB1376)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

Synthetic Peptide within Human Nitric oxide synthase, brain aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29475

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Reactivity data

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Product details

What is this antibody validated in?
Anti-nNOS (neuronal) antibody (ab1376) is a goat polyclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-nNOS (neuronal) (ab1376) was first used in a scientific publication in 2002 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-nNOS (neuronal) (ab1376) has over 20 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.

Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

See full target information Nitric oxide synthase, brain

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Publications (98)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:1146-1159 PubMed40442499

2025

The transcriptomes, connections and development of submucosal neuron classes in the mouse small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Khomgrit Morarach,Ziwei Liu,Sanghita Banerjee,Yanan Chen,Ashley L Harb,Joel M Kosareff,Charles R Hall,Fernando López-Redondo,Elham Jalalvand,Suad H Mohamed,Anastassia Mikhailova,David R Linden,Ulrika Marklund

Acta neuropathologica communications 13:58 PubMed40075409

2025

Assessment of gastrointestinal function and enteric nervous system changes over time in the A53T mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Myat Noe Han,Madeleine R Di Natale,Enie Lei,John B Furness,David I Finkelstein,Marlene M Hao,Shanti Diwakarla,Rachel M McQuade

Frontiers in molecular neuroscience 17:1481956 PubMed39640944

2024

Inhibition of midbrain cholinergic neurons impairs decision-making strategies during reversal learning.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwoong Kim,Nadine K Gut,Michael W Shiflett,Juan Mena-Segovia

Acta biochimica et biophysica Sinica 57:616-632 PubMed39544002

2024

Mechanism of RSL3-induced ferroptotic cell death in HT22 cells: crucial role of protein disulfide isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Jie Hou,Xuanqi Huang,Bao Ting Zhu

Scientific reports 14:23988 PubMed39402104

2024

Protection of HT22 neuronal cells against chemically-induced ferroptosis by catechol estrogens: protein disulfide isomerase as a mechanistic target.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanqi Huang,Ming-Jie Hou,Bao Ting Zhu

Cellular and molecular gastroenterology and hepatology 18:101405 PubMed39299667

2024

Interindividual Variation in Gut Nitrergic Neuron Density Is Regulated By GDNF Levels and ETV1.

Applications

Unspecified application

Species

Unspecified reactive species

Heikki T Virtanen,Peyman Choopanian,L Lauriina Porokuokka,Richard Forsgård,Daniel R Garton,Soophie Olfat,Riitta Korpela,Mehdi Mirzaie,Jaan-Olle Andressoo

The Journal of clinical investigation 134: PubMed39207863

2024

West Nile virus triggers intestinal dysmotility via T cell-mediated enteric nervous system injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Janova,Fang R Zhao,Pritesh Desai,Matthias Mack,Larissa B Thackray,Thaddeus S Stappenbeck,Michael S Diamond

PLoS pathogens 20:e1011766 PubMed39141685

2024

Helminth infection driven gastrointestinal hypermotility is independent of eosinophils and mediated by alterations in smooth muscle instead of enteric neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Haozhe Wang,Kristian Barry,Aidil Zaini,Gillian Coakley,Mati Moyat,Carmel P Daunt,Lakshanie C Wickramasinghe,Rossana Azzoni,Roxanne Chatzis,Bibek Yumnam,Mali Camberis,Graham Le Gros,Olaf Perdijk,Jaime P P Foong,Joel C Bornstein,Benjamin J Marsland,Nicola L Harris

Cell reports 43:113970 PubMed38512868

2024

Long-range inhibitory neurons mediate cortical neurovascular coupling.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine F Ruff,Fernanda Juarez Anaya,Samuel J Dienel,Adiya Rakymzhan,Alain Altamirano-Espinoza,Jonathan J Couey,Mitsuhiro Fukuda,Alan M Watson,Aihua Su,Kenneth N Fish,Maria E Rubio,Bryan M Hooks,Sarah E Ross,Alberto L Vazquez

Frontiers in neuroanatomy 17:1210502 PubMed38020216

2023

Secretagogin as a marker to distinguish between different neuron types in human frontal and temporal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Tapia-González,Javier DeFelipe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-nNOS (neuronal) antibody

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (AB1376)

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (AB1376)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (98)

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