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AB76067

Anti-nNOS (neuronal) antibody [EP1855Y]

5

(10 Reviews)

|

(93 Publications)

Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) is a rabbit monoclonal antibody detecting nNOS (neuronal) in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
- Trusted since 2009

View Alternative Names

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

9 Images
Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with unpurified ab76067 at 1/15 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Immunocytochemistry analysis of PC-12 cells labelling nNOS (neuronal) (green) with purified ab76067 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Overlay histogram showing PC-12 cells stained with unpurified ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76067, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC-12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Intracellular Flow Cytometry analysis of PC-12 cells labelling nNos (neuronal) with purified ab76067 at 1/600 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • IP

Unknown

Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

ab76067 (unpurified) at 1/4 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Predicted band size: 160 kDa

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Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • IP

Unknown

Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

ab76067 (purified) at 1/150 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067)

Predicted band size: 160 kDa

false

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • WB

Unknown

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

All lanes:

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 160 kDa

Observed band size: 161 kDa

false

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • WB

Lab

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/3000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 160 kDa

false

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)
  • WB

Lab

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (AB76067)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) at 1/100 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 160 kDa

false

  • Carrier free

    Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1855Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p><strong>For unpurified use at 1/100.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/150", "IP-species-notes": "<p><strong>For unpurified use at 1/4.</strong></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/15. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Common marmoset": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-nNOS (neuronal) antibody [EP1855Y] (ab76067) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.

What is the molecular weight of nNOS (neuronal)?
Anti-nNOS (neuronal) [EP1855Y] (ab76067) specifically detects a band for nNOS (neuronal) (UniProt: P29475) at a molecular weight of 161kDa.

Trusted by the scientific community
Anti-nNOS (neuronal) [EP1855Y] (ab76067) was first used in a scientific publication in 2009 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-nNOS (neuronal) [EP1855Y] (ab76067) has over 10 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP1855Y] also available for your convenience: ab76067, Alexa Fluor® 594 - ab198516, Carrier free - ab219373, Carrier free - ab271857

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
See full target information Nitric oxide synthase, brain
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Publications (93)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 15:e70728 PubMed40760806

2025

Curcumin Promotes the Recovery of Motor Function After Brachial Plexus Avulsion Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sijing Li,Bing He,Guijuan Zhou,Lin Wu,Xuanwei Wen,Limin Deng,Shudong Lin,Guozhi Liu,Shuangxi Chen,Zijian Xiao

European journal of pain (London, England) 29:e70047 PubMed40411263

2025

Astrocytic Inducible Nitric Oxide Synthase Upregulation Contributes to Chronic Below-Level Neuropathic Pain Following Spinal Cord Injury in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Youngkyung Kim,Hyunggoo Kang,Young Wook Yoon

Cell communication and signaling : CCS 23:218 PubMed40336106

2025

Strong protection by bazedoxifene against chemically-induced ferroptotic neuronal death in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Hao,Yifan Wang,Ming-Jie Hou,Yong Xiao Yang,Lixi Liao,Tongxiang Chen,Pan Wang,Xiaojun Chen,Bao Ting Zhu

Pharmaceutical biology 63:218-228 PubMed40205764

2025

, a standardized herbal formula, promotes gastrointestinal motility via modulation of the acetylcholine pathway in a loperamide-induced functional dyspepsia mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Ju Hwang,Chang-Seob Seo,Dong-Cheol Baek,Tae-Wook Woo,Jing-Hua Wang,Jin-Seok Lee,Yu-Jin Choi,Ji-Yeon Gu,Dong-Seon Kim,Chang-Gue Son

Frontiers in cell and developmental biology 13:1528972 PubMed40177131

2025

Arginase-II promotes melanoma and lung cancer cell growth by regulating Sirt3-mtROS axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Andrea Brenna,Duilio M Potenza,Santhoshkumar Sundaramoorthy,Xin Cheng,Xiu-Fen Ming,Zhihong Yang

PloS one 20:e0316798 PubMed39913351

2025

Nutraceutical COMP-4 confers protection against endothelial dysfunction through the eNOS/iNOS-NO-cGMP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Monica G Ferrini,Andrea Abraham,Revecca Millán,Leslie Graciano,Sriram V Eleswarapu,Jacob Rajfer

Journal of inflammation research 17:8945-8964 PubMed39588137

2024

Early-Life Stress Induced by Neonatal Maternal Separation Leads to Intestinal 5-HT Accumulation and Causes Intestinal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Yang,Rulan Bai,Chengzhong Li,Yan Sun,Hongyu Jing,Zixu Wang,Yaoxing Chen,Yulan Dong

Cells 13: PubMed39404390

2024

Intestinal Motility Dysfunction in Goto-Kakizaki Rats: Role of the Myenteric Plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Mandú Gimenes,Joice Naiara Bertaglia Pereira,Eliane Borges da Silva,Alef Aragão Carneiro Dos Santos,Thais Martins Rodrigues,Giovanna de Oliveira Santana,Maria Vitoria Martins Scervino,Tania Cristina Pithon-Curi,Sandro Massao Hirabara,Renata Gorjão,Rui Curi

Molecular neurobiology 62:3934-3955 PubMed39354232

2024

Raloxifene Prevents Chemically-Induced Ferroptotic Neuronal Death In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Hao,Yifan Wang,Ming-Jie Hou,Lixi Liao,Yong Xiao Yang,Ying-Hua Wang,Bao Ting Zhu

Frontiers in pharmacology 15:1411642 PubMed39139632

2024

The 5HT4R agonist velusetrag efficacy on neuropathic chronic intestinal pseudo-obstruction in PrP-SCA7-92Q transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Liu,Yunfei Wu,Dewan Ren,Yulong Tao,Fangyi Mai,Jingyi Zhu,Xiang Li,Emanuela Colla,Maria Grimaldi,Roberto Giovannini,Fabrizio Giorgi,Loredana Vesci
View all publications
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Product promise

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