Rabbit Polyclonal nNOS antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nos1.
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- Oxidative medicine and cellular longevity 2022:82960432022Mechanisms of NO-Mediated Protein S-Nitrosylation in the Lens-Induced Myopia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Lu,Weitao Song,Yuanjun Li,Jingge Xiao,Kaixuan Du,Qiuman Fu,Yanni Zhang,Liting Zhao,Yewei Yin,Tu Hu,Dan WenPubMed 36439692
- Science advances 8:eabm14442022Novel oxygen sensing mechanism in the spinal cord involved in cardiorespiratory responses to hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole O Barioni,Fatemeh Derakhshan,Luana Tenorio Lopes,Hiroshi Onimaru,Arijit Roy,Fiona McDonald,Erika Scheibli,Mufaddal I Baghdadwala,Negar Heidari,Manisha Bharadia,Keiko Ikeda,Itaru Yazawa,Yasumasa Okada,Michael B Harris,Mathias Dutschmann,Richard J A WilsonPubMed 35333571
- American journal of physiology. Gastrointestinal and liver physiology 319:G481-G4932020Inhibition of GSK-3β restores delayed gastric emptying in obesity-induced diabetic female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChethan Sampath,Shanthi Srinivasan,Michael L Freeman,Pandu R GangulaPubMed 32812777
- Cerebral cortex (New York, N.Y. : 1991) 30:2128-21432019AMPA Induces NO-Dependent cGMP Signals in Hippocampal and Cortical Neurons via L-Type Voltage-Gated Calcium Channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJan Giesen,Ernst-Martin Füchtbauer,Annette Füchtbauer,Klaus Funke,Doris Koesling,Michael RusswurmPubMed 31711126
- Digestive diseases and sciences 64:3502-35172019Impairment of Nrf2- and Nitrergic-Mediated Gastrointestinal Motility in an MPTP Mouse Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Sampath et. al.PubMed 31187328
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Nos1. The exact immunogen used to generate this antibody is proprietary information. Database link P29476
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Expected | Tested |
| Rat | Tested | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
Alternative names
Bnos, Nos1, Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS
Recommended products
Rabbit Polyclonal nNOS antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nos1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Nos1. The exact immunogen used to generate this antibody is proprietary information. Database link P29476
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
- Biological function summary
NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.
- Pathways
NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.
- Associated diseases and disorders
NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
5% SDS-PAGE
All lanes: Western blot - Anti-nNOS (neuronal) antibody - N-terminal (ab229785) at 1/1000 dilution
Lane 1: Mouse brain tissue extracts at 50 µg
Lane 2: Rat brain tissue extracts at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 160 kDa
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
DIV9 rat E18 primary cortical neurons cells stained for nNOS (neuronal) (green) using ab229785 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstains: DAPI (blue) and beta Tubulin 3/Tuj1 (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
Paraffin-embedded rat brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
Frozen-sectioned adult mouse cerebellum tissue stained for nNOS (neuronal) with ab229785 at 1/250 dilution in immunohistochemical analysis. NF-H (red), DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
Paraffin-embedded mouse brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.
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