AB229785

Anti-nNOS (neuronal) antibody - N-terminal

Name: Anti-nNOS (neuronal) antibody - N-terminal (ab229785) | Abcam
Brand: Abcam Limited
SKU: AB229785
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal nNOS antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nos1.

View Alternative Names

Bnos, Nos1, Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Paraffin-embedded rat brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Frozen-sectioned adult mouse cerebellum tissue stained for nNOS (neuronal) with ab229785 at 1/250 dilution in immunohistochemical analysis. NF-H (red), DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Paraffin-embedded mouse brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

DIV9 rat E18 primary cortical neurons cells stained for nNOS (neuronal) (green) using ab229785 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstains : DAPI (blue) and beta Tubulin 3/Tuj1 (red).

Supplier Data

Western blot - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

5% SDS-PAGE

All lanes:

Western blot - Anti-nNOS (neuronal) antibody - N-terminal (ab229785) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 160 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

ICC/IF, IHC-Fr, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Nos1. The exact immunogen used to generate this antibody is proprietary information.

P29476

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.

Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.

See full target information Nos1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:8296043 PubMed36439692

2022

Mechanisms of NO-Mediated Protein S-Nitrosylation in the Lens-Induced Myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lu,Weitao Song,Yuanjun Li,Jingge Xiao,Kaixuan Du,Qiuman Fu,Yanni Zhang,Liting Zhao,Yewei Yin,Tu Hu,Dan Wen

Science advances 8:eabm1444 PubMed35333571

2022

Novel oxygen sensing mechanism in the spinal cord involved in cardiorespiratory responses to hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole O Barioni,Fatemeh Derakhshan,Luana Tenorio Lopes,Hiroshi Onimaru,Arijit Roy,Fiona McDonald,Erika Scheibli,Mufaddal I Baghdadwala,Negar Heidari,Manisha Bharadia,Keiko Ikeda,Itaru Yazawa,Yasumasa Okada,Michael B Harris,Mathias Dutschmann,Richard J A Wilson

PeerJ 9:e11512 PubMed34141479

2021

Neuromedin U and neurotensin may promote the development of the tumour microenvironment in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daheng Yang,Xianwei Zhang,Zheqian Li,Fei Xu,Chenjie Tang,Hongbing Chen

American journal of physiology. Gastrointestinal and liver physiology 319:G481-G493 PubMed32812777

2020

Inhibition of GSK-3β restores delayed gastric emptying in obesity-induced diabetic female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chethan Sampath,Shanthi Srinivasan,Michael L Freeman,Pandu R Gangula

Cerebral cortex (New York, N.Y. : 1991) 30:2128-2143 PubMed31711126

2019

AMPA Induces NO-Dependent cGMP Signals in Hippocampal and Cortical Neurons via L-Type Voltage-Gated Calcium Channels.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Giesen,Ernst-Martin Füchtbauer,Annette Füchtbauer,Klaus Funke,Doris Koesling,Michael Russwurm

Digestive diseases and sciences 64:3502-3517 PubMed31187328

2019

Impairment of Nrf2- and Nitrergic-Mediated Gastrointestinal Motility in an MPTP Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

C Sampath,R Kalpana,T Ansah,C Charlton,A Hale,K M Channon,S Srinivasan,P R Gangula

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-nNOS (neuronal) antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Western blot - Anti-nNOS (neuronal) antibody - N-terminal (AB229785)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

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Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise