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AB5583

Anti-nNOS (neuronal) (phospho S1417) antibody

3

(6 Reviews)

|

(28 Publications)

Rabbit Polyclonal nNOS phospho S1417 antibody. Suitable for ICC, IP, WB, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human, Rabbit samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human Nitric oxide synthase, brain phospho S1417 aa 1400 to C-terminus.

View Alternative Names

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (AB5583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (AB5583)

Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1 : 20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Western blot - Anti-nNOS (neuronal) (phospho S1417) antibody (AB5583)
  • WB

Supplier Data

Western blot - Anti-nNOS (neuronal) (phospho S1417) antibody (AB5583)

All lanes:

Western blot - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583) at 1/1000 dilution

Lane 1:

untreated SH-SY5Y cells (Human neuroblastoma cell line from bone marrow) at 20 µg

Lane 2:

SH-SY5Y cells (Human neuroblastoma cell line from bone marrow) treated with 100 ng/mL nocodazole at 20 µg

Secondary

All lanes:

HRP-conjugated secondary antibody

Predicted band size: 160 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse, Rabbit

Applications

WB, IP, ICC, IHC-Fr, ICC/IF, IHC-FoFr

applications

Immunogen

Synthetic Peptide within Human Nitric oxide synthase, brain phospho S1417 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29475

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
See full target information Nitric oxide synthase, brain phospho S1417
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:58 PubMed40555714

2025

Sleep prevents brain phosphoproteome disruption to safeguard survival.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Juhang Liu,Yu Li,Yikui Zhao,Yu Tian,Bing Hu,Kaiyue Yan,Ying Li,Kaihang Ding,Xiangyu Wang,Huiwen Tian,Wen Si,Ketong Liu,Huiran Zhang,Chongchong Zhao,Guangfu Wang,Zhiqiang Wang

Scientific reports 15:15205 PubMed40307298

2025

Glutamate gradually elevates [Zn] via the CaM-CaMKII-NOS cascade in primary cultured rat embryonic cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chiun Tseng,Yong-Sheng Wang,Chien-Yuan Pan

Function (Oxford, England) 5: PubMed39289003

2024

A Skeletal Muscle-Mediated Anticontractile Response on Vascular Tone: Unraveling the Lactate-AMPK-NOS1 Pathway in Femoral Arteries.

Applications

Unspecified application

Species

Unspecified reactive species

Milene T Fontes,Tiago J Costa,Ricardo B de Paula,Fênix A Araújo,Paula R Barros,Paul Townsend,Landon Butler,Kandy T Velazquez,Fiona Hollis,Gisele F Bomfim,Joshua T Butcher,Cameron G McCarthy,Camilla F Wenceslau

Cell 186:5812-5825.e21 PubMed38056462

2023

An enzyme that selectively S-nitrosylates proteins to regulate insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Lin Zhou,Zachary W Grimmett,Nicholas M Venetos,Colin T Stomberski,Zhaoxia Qian,Precious J McLaughlin,Puneet K Bansal,Rongli Zhang,James D Reynolds,Richard T Premont,Jonathan S Stamler

Endocrinology 164: PubMed37823477

2023

Ghrelin Action in the PVH of Male Mice: Accessibility, Neuronal Targets, and CRH Neurons Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gimena Fernandez,Pablo N De Francesco,María P Cornejo,Agustina Cabral,Julieta P Aguggia,Victor J Duque,Nilufer Sayar,Sonia Cantel,Juan I Burgos,Jean-Alain Fehrentz,Rodrigo Rorato,Deniz Atasoy,André S Mecawi,Mario Perello

Molecular neurobiology 61:148-166 PubMed37589832

2023

2-Methoxyestradiol and Hydrogen Peroxide as Promising Biomarkers in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Bastian,Lucyna Konieczna,Jarosław Dulski,Agnieszka Daca,Dagmara Jacewicz,Agata Płoska,Narcyz Knap,Jarosław Sławek,Tomasz Bączek,Leszek Kalinowski,Joanna Drzeżdżon,Anna Roszmann,Mariusz Belka,Magdalena Górska-Ponikowska

Science advances 9:eade5584 PubMed36753544

2023

Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yizheng Wu,Shuying Shen,Jiaxin Chen,Weiyu Ni,Qinxin Wang,Hongyi Zhou,Junxin Chen,Haitao Zhang,Zixuan Mei,Xuewu Sun,Panyang Shen,Zhiwei Jie,Wenbin Xu,Zhenghua Hong,Yan Ma,Kefan Wang,Shuanglin Wan,Hongfei Wu,Ziang Xie,An Qin,Shunwu Fan

Antioxidants (Basel, Switzerland) 11: PubMed36290736

2022

2-Methoxyestradiol Damages DNA in Glioblastoma Cells by Regulating nNOS and Heat Shock Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Emilia Bastian,Agnieszka Daca,Agata Płoska,Alicja Kuban-Jankowska,Leszek Kalinowski,Magdalena Gorska-Ponikowska

PloS one 15:e0243660 PubMed33296434

2020

Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Munehiro Uda,Toshinori Yoshihara,Noriko Ichinoseki-Sekine,Takeshi Baba,Toshitada Yoshioka

Journal of neuroinflammation 17:185 PubMed32532282

2020

CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chiu-Yi Ho,Yu-Te Lin,Hsin-Hung Chen,Wen-Yu Ho,Gwo-Ching Sun,Michael Hsiao,Pei-Jung Lu,Pei-Wen Cheng,Ching-Jiunn Tseng
View all publications
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