Rabbit Polyclonal nNOS phospho S847 antibody. Suitable for IHC-FoFr, WB and reacts with Rat, Mouse, Human samples. Cited in 15 publications.
Images
Publications
Filter by
- PloS one 15:e02436602020Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMunehiro Uda et. al.PubMed 33296434
- Brain research bulletin 165:272-2802020Effects of chronic ethanol consumption on the expression of GLT-1 and neuroplasticity-related proteins in the nucleus accumbens of alcohol-preferring rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHasan Alhaddad,Fawaz Alasmari,Balsam Alhamadani,Woonyen Wong,Richard L Bell,Youssef SariPubMed 33075417
- Nitric oxide : biology and chemistry 87:73-822019Exercise training augments neuronal nitric oxide synthase dimerization in the paraventricular nucleus of rats with chronic heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeeru M Sharma et. al.PubMed 30878404
- The Journal of neuroscience : the official journal of the Society for Neuroscience 37:742-7562017Accumbens nNOS Interneurons Regulate Cocaine Relapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander C W Smith,Michael D Scofield,Jasper A Heinsbroek,Cassandra D Gipson,Daniela Neuhofer,Doug J Roberts-Wolfe,Sade Spencer,Constanza Garcia-Keller,Neringa M Stankeviciute,Rachel J Smith,Nicholas P Allen,Melissa R Lorang,William C Griffin,Heather A Boger,Peter W KalivasPubMed 28123012
- Cephalalgia : an international journal of headache 37:1350-13722016Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank L Rice,Jennifer Y Xie,Phillip J Albrecht,Emily Acker,Justin Bourgeois,Edita Navratilova,David W Dodick,Frank PorrecaPubMed 27852962
- Biological & pharmaceutical bulletin 39:1922-19312016Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheu-Ran Choi et. al.PubMed 27601184
- PloS one 9:e987132014β3-Adrenoreceptor stimulation protects against myocardial infarction injury via eNOS and nNOS activation.
- The Journal of pharmacology and experimental thera 339:367-752011Role of neuronal nitric-oxide synthase in estrogen-induced relaxation in rat resistance arteries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Lekontseva et. al.PubMed 21807885
- Antioxidants & redox signaling 15:873-872011RGSZ2 binds to the neural nitric oxide synthase PDZ domain to regulate mu-opioid receptor-mediated potentiation of the N-methyl-D-aspartate receptor-calmodulin-dependent protein kinase II pathway.
- British journal of pharmacology 163:1707-202011Spinal neuronal NOS activation mediates sigma-1 receptor-induced mechanical and thermal hypersensitivity in mice: involvement of PKC-dependent GluN1 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDae-Hyun Roh,Sheu-Ran Choi,Seo-Yeon Yoon,Suk-Yun Kang,Ji-Young Moon,Soon-Gu Kwon,Ho-Jae Han,Alvin J Beitz,Jang-Hern LeePubMed 21391983
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-FoFr | WB | |
|---|---|---|
| Human | Expected | Expected |
| Mouse | Expected | Tested |
| Rat | Tested | Expected |
| Apteronotus leptorhynchus | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Xenopus laevis, Zebrafish, Apteronotus leptorhynchus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Xenopus laevis, Zebrafish, Apteronotus leptorhynchus | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.
Alternative names
Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, Nos1
Recommended products
Rabbit Polyclonal nNOS phospho S847 antibody. Suitable for IHC-FoFr, WB and reacts with Rat, Mouse, Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody shows a reduction in signal when blocked with unmodified nNOS (neuronal) peptide in WB, however when tested in ELISA, it showed less than 2% cross reactivity with the unmodified protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
- Biological function summary
NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.
- Pathways
NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.
- Associated diseases and disorders
NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16650 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/mL
Lane 1: Forebrain (Mouse) Tissue Lysate at 20 µg
Lane 2: Spinal Cord (Mouse) Tissue Lysate at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) at 20 µg
Lane 4: Forebrain (Mouse) Tissue Lysate at 20 µg with Mouse nNOS (neuronal) (phospho S847) peptide
Lane 5: Spinal Cord (Mouse) Tissue Lysate at 20 µg with Mouse nNOS (neuronal) (phospho S847) peptide
Lane 6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) at 20 µg with Mouse nNOS (neuronal) (phospho S847) peptide
Lane 7: Forebrain (Mouse) Tissue Lysate at 20 µg with Mouse nNOS (neuronal) (unmodified) peptide
Lane 8: Spinal Cord (Mouse) Tissue Lysate at 20 µg with Mouse nNOS (neuronal) (unmodified) peptide
Lane 9: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) at 20 µg with Mouse nNOS (neuronal) (unmodified) peptide
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDa, 55 kDa
Exposure time: 4min
Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
All lanes: Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/mL
Lane 1: Forebrain (Mouse) Tissue Lysate at 20 µg
Lane 2: Spinal Cord (Mouse) Tissue Lysate at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDa
Exposure time: 2min
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomWestern blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
Lanes 1 - 3: Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/mL
Lane 4: nNOS antibody at 1/2500 dilution
Lanes 1 and 4: mouse forebrain at 20 µg
Lane 2: mouse forebrain at 20 µg with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981)
Lane 3: mouse forebrain at 20 µg with corresponding unmodified nNOS (neuronal) peptide
Predicted band size: 160 kDa
Observed band size: 160 kDa
This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistry (PFA perfusion fixed frozen sections) - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
Immunostaining using Rabbit polyclonal to nNOS (neuronal) (phospho S847) (ab16650) on rat brain tissue sections (30 micron free floating). ab16650 was used at a dilution of 1/3000 and incubated for 18 hours at RT (in PBS triton 0.3%). Secondary Antibody Goat anti-rabbit alexa Fluor 488
was used at a dilution of 1/1000. The image shows cytoplasmic staining of CNS neurons with ab16650 in naïve rats; the staining being observed in the soma and processes of these neurons. The staining was quenched by pre-incubation with peptide against phospho S847 (ab16981), but not by the control peptide (ab57047) indicating that ab16650 is specific for nNos phosphorylated at S847.
Protocol: Rats were perfused-fixed with 4% paraformaldehyde. Tissues were post-fixed overnight in the same fixative and then cryoprotected in 20% sucrose overnight. Following embedding in OCT and freezing, tissues were cut and immunostained using the 'free floating’ technique.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com