JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB110352

Anti-NNT antibody [8B4BB10]

4

(2 Reviews)

|

(10 Publications)

Mouse Monoclonal NNT antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 10 publications.

View Alternative Names

Nicotinamide nucleotide transhydrogenase, Pyridine nucleotide transhydrogenase, NNT

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10] (AB110352)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10] (AB110352)

Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.

Flow Cytometry - Anti-NNT antibody [8B4BB10] (AB110352)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-NNT antibody [8B4BB10] (AB110352)

HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT antibody [8B4BB10] (AB110352)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT antibody [8B4BB10] (AB110352)

NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Immunoprecipitation - Anti-NNT antibody [8B4BB10] (AB110352)
  • IP

Unknown

Immunoprecipitation - Anti-NNT antibody [8B4BB10] (AB110352)

Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.

All lanes:

Immunoprecipitation - Anti-NNT antibody [8B4BB10] (ab110352)

Predicted band size: 114 kDa

false

Western blot - Anti-NNT antibody [8B4BB10] (AB110352)
  • WB

Unknown

Western blot - Anti-NNT antibody [8B4BB10] (AB110352)

anti-Complex II 30kDa subunit has also been labeled as a control.

All lanes:

Western blot - Anti-NNT antibody [8B4BB10] (ab110352) at 0.5 µg/mL

All lanes:

HepG2 whole cell lysate

Predicted band size: 114 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B4BB10

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, IP, Flow Cyt, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Several customers have found that this antibody may not work well for mouse and rat samples.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicotinamide nucleotide transhydrogenase (NNT) is an enzyme that plays an important role in cellular redox balance by catalyzing the hydride transfer between NADH and NADP+ across the inner mitochondrial membrane. Also known as NNT in common scientific references the enzyme has a mass of approximately 109 kDa. NNT is primarily expressed in tissues with high metabolic demand such as the liver heart and adrenal glands. Its high expression in the mitochondria reflects its essential role in maintaining efficient energy homeostasis.
Biological function summary

NNT regulates the conversion of NADP+ to NADPH a form that serves as an important reducing agent in cellular mechanisms. The enzyme does not function alone but integrates into the mitochondrial membrane working as a part of a complex with other mitochondrial proteins like ATP synthase and cytochrome oxidase. This role is important for antioxidant defense as NADPH is utilized in pathways that mitigate reactive oxygen species maintaining cellular integrity.

Pathways

NNT is a central component in energy metabolism and detoxification processes. It is intricately involved in the pentose phosphate pathway where it helps generate reducing equivalents in the form of NADPH. Additionally NNT is linked to the oxidative phosphorylation pathway ensuring efficient ATP production. The enzyme's activity is closely associated with proteins such as glutathione reductase and thioredoxin which rely on the reducing power of NADPH provided by NNT for their antioxidant functions.

NNT mutations have been associated with familial glucocorticoid deficiency and metabolic disorders which highlight its importance in endocrine and energy balance. Altered NNT activity has been observed in various cases of hypertension and heart failure. The enzyme's interrelationship with proteins like cortisol which is important in adrenal function and insulin in relation to metabolic processes and regulation indicate its broad influence in both energy homeostasis and redox balance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane (By similarity). May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland (PubMed : 22634753).
See full target information NNT
style
left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4244 PubMed38762605

2024

Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan P Ward,Sang Jun Yoon,Tyce Flynn,Amanda M Sherwood,Maddison A Olley,Juliana Madej,Gina M DeNicola

American journal of physiology. Renal physiology 323:F92-F106 PubMed35499238

2022

Regulation of kidney mitochondrial function by caloric restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Julian D C Serna,Andressa G Amaral,Camille C Caldeira da Silva,Ana C Munhoz,Eloisa A Vilas-Boas,Sergio L Menezes-Filho,Alicia J Kowaltowski

Frontiers in molecular neuroscience 14:688050 PubMed34630032

2021

Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.

Applications

Unspecified application

Species

Unspecified reactive species

Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen Liu

Cell 184:4268-4283.e20 PubMed34233163

2021

NNT mediates redox-dependent pigmentation via a UVB- and MITF-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Allouche,Inbal Rachmin,Kaustubh Adhikari,Luba M Pardo,Ju Hee Lee,Alicia M McConnell,Shinichiro Kato,Shaohua Fan,Akinori Kawakami,Yusuke Suita,Kazumasa Wakamatsu,Vivien Igras,Jianming Zhang,Paula P Navarro,Camila Makhlouta Lugo,Haley R Noonan,Kathleen A Christie,Kaspar Itin,Nisma Mujahid,Jennifer A Lo,Chong Hyun Won,Conor L Evans,Qing Yu Weng,Hequn Wang,Sam Osseiran,Alyssa Lovas,István Németh,Antonio Cozzio,Alexander A Navarini,Jennifer J Hsiao,Nhu Nguyen,Lajos V Kemény,Othon Iliopoulos,Carola Berking,Thomas Ruzicka,Rolando Gonzalez-José,Maria-Cátira Bortolini,Samuel Canizales-Quinteros,Victor Acuna-Alonso,Carla Gallo,Giovanni Poletti,Gabriel Bedoya,Francisco Rothhammer,Shosuke Ito,Maria Vittoria Schiaffino,Luke H Chao,Benjamin P Kleinstiver,Sarah Tishkoff,Leonard I Zon,Tamar Nijsten,Andrés Ruiz-Linares,David E Fisher,Elisabeth Roider

Cell metabolism 32:44-55.e6 PubMed32402267

2020

Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Leena P Bharath,Madhur Agrawal,Grace McCambridge,Dequina A Nicholas,Hatice Hasturk,Jing Liu,Kai Jiang,Rui Liu,Zhenheng Guo,Jude Deeney,Caroline M Apovian,Jennifer Snyder-Cappione,Gregory S Hawk,Rebecca M Fleeman,Riley M F Pihl,Katherine Thompson,Anna C Belkina,Licong Cui,Elizabeth A Proctor,Philip A Kern,Barbara S Nikolajczyk

The Journal of experimental medicine 217: PubMed32196080

2020

Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan P Ward,Yun Pyo Kang,Aimee Falzone,Theresa A Boyle,Gina M DeNicola

Biomolecules 9: PubMed30823587

2019

Saturated Fatty Acid Activates T Cell Inflammation Through a Nicotinamide Nucleotide Transhydrogenase (NNT)-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Grace McCambridge,Madhur Agrawal,Alanna Keady,Philip A Kern,Hatice Hasturk,Barbara S Nikolajczyk,Leena P Bharath

Molecules (Basel, Switzerland) 23: PubMed30041436

2018

Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method.

Applications

Unspecified application

Species

Unspecified reactive species

Weiquan Ren,Sheng Gao,Huimin Zhang,Yinglu Ren,Xue Yu,Weili Lin,Shuzhen Guo,Ruixin Zhu,Wei Wang

The Journal of biological chemistry 291:11185-97 PubMed27048652

2016

Compartment-specific Control of Reactive Oxygen Species Scavenging by Antioxidant Pathway Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Dey,Agnieszka Sidor,Brian O'Rourke

American journal of physiology. Lung cellular and molecular physiology 303:L889-98 PubMed23064950

2012

Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.

Applications

WB

Species

Mouse

Amit R Agarwal,Liqin Zhao,Harsh Sancheti,Isaac K Sundar,Irfan Rahman,Enrique Cadenas
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com