JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238853

Anti-NNT1 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal NNT1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CLCF1 aa 1-200.

View Alternative Names

BSF3, CLC, NNT1, CLCF1, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, Novel neurotrophin-1, BSF-3, NNT-1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT1 antibody (AB238853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT1 antibody (AB238853)

Paraffin-embedded human kidney tissue stained for NNT1 using ab238853 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CLCF1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UBD9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'NNT1' also known as nicotinamide nucleotide transhydrogenase is a mitochondrial enzyme with a mass of approximately 114 kDa. It is expressed in various tissues with high concentrations in the heart liver and kidney. Mechanically NNT1 is a membrane-bound enzyme that acts to transfer hydride ions between NADH and NADP+ facilitating the production of NADPH within the mitochondria. This process is important for maintaining cellular redox balance and supporting antioxidant defense systems.
Biological function summary

The function of maintaining NADPH levels makes NNT1 an important player in protecting cells from oxidative damage. It functions as part of the inner mitochondrial membrane and is not generally considered to be part of a larger complex in its basic form. By perpetuating a pool of available NADPH NNT1 aids cellular processes such as lipid synthesis and detoxification.

Pathways

The activity of NNT1 is integral to the maintenance of mitochondrial homeostasis particularly the oxidative phosphorylation pathway. It has associations with the glutathione antioxidant pathway as it contributes to the regeneration of reduced glutathione through provision of NADPH. The pathways involving NNT1 connect it to proteins such as glutathione reductase and those involved in the electron transport chain.

NNT1 has connections to metabolic disorders and conditions related to oxidative stress. Mutations in the NNT1 gene can be linked to familial glucocorticoid deficiency a condition affecting adrenal gland function. This protein is also related to oxidative stress which can connect it to neurodegenerative disorders with ties to proteins involved in mitochondrial dysfunction and stress responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
See full target information CLCF1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB32101

Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

  • 3
  • 4
Primary Antibodies

AB110352

Anti-NNT antibody [8B4BB10]

Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10] (AB110352)

  • 4
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB137332

Anti-MMP1 antibody

Western blot - Anti-MMP1 antibody (AB137332)

  • 4
  • 12

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com