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AB224619

Anti-NOB1 antibody

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(4 Publications)

Rabbit Polyclonal NOB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NOB1 aa 100-250.

View Alternative Names

ART4, NOB1P, PSMD8BP1, MSTP158, NOB1, RNA-binding protein NOB1, Phosphorylation regulatory protein HP-10, Protein ART-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (AB224619)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (AB224619)

Paraffin-embedded human thyroid tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (AB224619)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOB1 antibody (AB224619)

Paraffin-embedded human placenta tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOB1 antibody (AB224619)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOB1 antibody (AB224619)

A549 (human lung carcinoma cell line) cells stained for NOB1 (green) using ab224619 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-NOB1 antibody (AB224619)
  • WB

Supplier Data

Western blot - Anti-NOB1 antibody (AB224619)

All lanes:

Western blot - Anti-NOB1 antibody (ab224619) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Mouse stomach tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NOB1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9ULX3

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolar protein 1 (NOB1) plays an important role in ribosomal RNA processing and biogenesis. Often referred to as NOB1p it is a protein that weighs approximately 55 kDa. NOB1 is expressed mainly in the nucleolus. This protein contains several domains such as the PIN domain which is important in its RNA cleavage function.
Biological function summary

NOB1 functions as a part of a complex called the 26S proteasome regulatory complex. It participates in the maturation of the 20S proteasome facilitating the processing of 18S rRNA which is important for ribosome assembly. This role highlights its importance for maintaining ribosomal function and protein synthesis.

Pathways

NOB1's role in ribosome assembly ties it to the ribosome biogenesis and protein synthesis pathways. It works closely with proteins such as PNO1 and the 18S rRNA processing machinery. These interactions ensure that ribosomes are properly assembled and functional which is critical for cellular growth and division.

Mutations or dysregulation of NOB1 have implications in cancer development. Aberrant expression of NOB1 has been linked to tumorigenesis particularly in pathways involving the oncogenic protein MYC. By influencing ribosome biogenesis NOB1 can contribute to the uncontrolled proliferation characteristic of cancer cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity).
See full target information NOB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 14:1389-1406 PubMed35150481

2022

Sublethal heat treatment promotes breast cancer metastasis and its molecular mechanism revealed by quantitative proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Shujun Xia,Xiaoyu Li,Shangyan Xu,Xiaofeng Ni,Weiwei Zhan,Wei Zhou

Frontiers in pharmacology 10:449 PubMed31068824

2019

LncRNA OIP5-AS1 Promotes the Proliferation of Hemangioma Vascular Endothelial Cells via Regulating miR-195-5p/NOB1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayu Zhang,Tiancheng Zhao,Leilei Tian,Yezhou Li

Molecular medicine reports 19:1903-1910 PubMed30628685

2019

MicroRNA‑744 suppresses cell proliferation and invasion of papillary thyroid cancer by directly targeting NOB1.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Liu,Jing Guo,Hongyan Chai,Xiangfeng Meng

American journal of cancer research 8:2238-2253 PubMed30555741

2018

microRNA-744 is downregulated in glioblastoma and inhibits the aggressive behaviors by directly targeting NOB1.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Deng,Yue Li,Qi Fang,Honghai Luo,Gang Zhu
View all publications
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Product promise

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