AB100880

Anti-Nociceptin antibody

Name: Anti-Nociceptin antibody (ab100880) | Abcam
Brand: Abcam Limited
SKU: AB100880
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Nociceptin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PNOC aa 100-150.

View Alternative Names

OFQ, PNOC, Prepronociceptin

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB100880)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB100880)

Dual labeling of Nociceptin (green) and enkephalin (red). 7 μm paraffin sections of human brain tissue were cut and floated onto positively charged slides. After being dewaxed and microwaved in 10mM citric acid (pH 6.0) for 20 minutes at 600W, sections were incubated with the rabbit polyclonal antisera overnight at 4°C, primary antibody was detected using biotinylated goat anti-rabbit IgG followed by incubation with peroxidase-conjugated avidin.

Binding of the primary antibody was visualized using 3-amino-9-ethylcarbazole (AEC) in acetate buffer. Afterwards, sections were rinsed, counterstained with Mayer`s hematoxylin and mounted in Vectamount mounting medium.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PNOC aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13519

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nociceptin also known as Orphanin FQ is a neuropeptide that functions as a ligand for the nociceptin receptor (NOP receptor or ORL1). It is a peptide made of 17 amino acids with an approximate mass of 2 kDa. Nociceptin is widely expressed in the central and peripheral nervous systems including the brain spinal cord and peripheral sensory neurons. It is involved in modulating various physiological processes through interaction with its receptor influencing neuronal excitability and synaptic plasticity.

Biological function summary

Nociceptin impacts pain modulation stress response and behavioral regulation. It forms a signaling complex with the NOP receptor which is a member of the opioid receptor family. Unlike classical opioid peptides nociceptin does not bind to opioid receptors (mu delta or kappa) and is considered to have a distinct role in the nervous system. Its action can be anti-opioid or analgesic depending on the context of its expression and the physiological state influenced.

Pathways

Nociceptin plays a significant role in nociceptive pathways and stress-related pathways. In the nociceptive pathways it regulates pain transmission through its interaction with the NOP receptor which can inhibit neurotransmitter release and hyperalgesia. Related proteins in these pathways include neurotransmitters like substance P and calcitonin gene-related peptide (CGRP). In stress-related pathways nociceptin interacts with components involved in the hypothalamic-pituitary-adrenal (HPA) axis modulating stress hormone release and affecting behavioral responses to stress.

Nociceptin has been linked to chronic pain conditions and anxiety disorders. Its dysregulation can contribute to altered pain perception seen in chronic pain syndromes with connections to proteins like cyclooxygenase (COX) in the peripheral inflammation response. In anxiety disorders nociceptin's influence on behavioral responses to stress relates to pathways involving corticotropin-releasing factor (CRF) which also interacts with the HPA axis. By understanding these connections researchers aim to explore nociceptin as a potential therapeutic target for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Nociceptin. Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.. Nocistatin. Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.. Orphanin FQ2. Has potent analgesic activity.

See full target information PNOC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genomics, proteomics & bioinformatics 19:754-771 PubMed33631425

2021

Oleic Acid and Eicosapentaenoic Acid Reverse Palmitic Acid-induced Insulin Resistance in Human HepG2 Cells via the Reactive Oxygen Species/JUN Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Sun,Jifeng Wang,Xiaojing Guo,Nali Zhu,Lili Niu,Xiang Ding,Zhensheng Xie,Xiulan Chen,Fuquan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nociceptin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB100880)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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