AB216413

Anti-Nociceptin antibody

Name: Anti-Nociceptin antibody (ab216413) | Abcam
Brand: Abcam Limited
SKU: AB216413

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(3 Publications)

Rabbit Polyclonal Nociceptin antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PNOC aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

OFQ, PNOC, Prepronociceptin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB216413)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB216413)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Nociceptin with ab216413 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Nociceptin antibody (AB216413)

15% gel.

All lanes:

Western blot - Anti-Nociceptin antibody (ab216413) at 1/200 dilution

Lane 1:

rat brain lysate

Lane 2:

rat liver lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PNOC aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13519

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nociceptin also known as Orphanin FQ is a neuropeptide that functions as a ligand for the nociceptin receptor (NOP receptor or ORL1). It is a peptide made of 17 amino acids with an approximate mass of 2 kDa. Nociceptin is widely expressed in the central and peripheral nervous systems including the brain spinal cord and peripheral sensory neurons. It is involved in modulating various physiological processes through interaction with its receptor influencing neuronal excitability and synaptic plasticity.

Biological function summary

Nociceptin impacts pain modulation stress response and behavioral regulation. It forms a signaling complex with the NOP receptor which is a member of the opioid receptor family. Unlike classical opioid peptides nociceptin does not bind to opioid receptors (mu delta or kappa) and is considered to have a distinct role in the nervous system. Its action can be anti-opioid or analgesic depending on the context of its expression and the physiological state influenced.

Pathways

Nociceptin plays a significant role in nociceptive pathways and stress-related pathways. In the nociceptive pathways it regulates pain transmission through its interaction with the NOP receptor which can inhibit neurotransmitter release and hyperalgesia. Related proteins in these pathways include neurotransmitters like substance P and calcitonin gene-related peptide (CGRP). In stress-related pathways nociceptin interacts with components involved in the hypothalamic-pituitary-adrenal (HPA) axis modulating stress hormone release and affecting behavioral responses to stress.

Nociceptin has been linked to chronic pain conditions and anxiety disorders. Its dysregulation can contribute to altered pain perception seen in chronic pain syndromes with connections to proteins like cyclooxygenase (COX) in the peripheral inflammation response. In anxiety disorders nociceptin's influence on behavioral responses to stress relates to pathways involving corticotropin-releasing factor (CRF) which also interacts with the HPA axis. By understanding these connections researchers aim to explore nociceptin as a potential therapeutic target for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nociceptin. Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.. Nocistatin. Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.. Orphanin FQ2. Has potent analgesic activity.

See full target information PNOC

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 13: PubMed39200401

2024

Ex Vivo Study of Colon Health, Contractility and Innervation in Male and Female Rats after Regular Exposure to Instant Cascara Beverage.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Gallego-Barceló,David Benítez-Álvarez,Ana Bagues,Blanca Silván-Ros,Alba Montalbán-Rodríguez,Laura López-Gómez,Gema Vera,María Dolores Del Castillo,José A Uranga,Raquel Abalo

Translational neuroscience 12:523-532 PubMed34963819

2021

Neuropeptide changes in an improved migraine model with repeat stimulations.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Guo,Yawen Cheng,Jiaqi An,Yi Qi,Guogang Luo

BMC neuroscience 22:25 PubMed33836649

2021

N/OFQ modulates orofacial pain induced by tooth movement through CGRP-dependent pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yan,Han Han,Shizhen Zhang,Yanzhu Lu,Linghuan Ren,Yufei Tang,Xiaolong Li,Fan Jian,Yan Wang,Hu Long,Wenli Lai

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nociceptin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nociceptin antibody (AB216413)

Western blot - Anti-Nociceptin antibody (AB216413)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise