AB238676

Anti-NOD1 antibody [EPR20833] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NOD1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Nucleotide-binding oligomerization domain-containing protein 1, mNod1, Nod1

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Supplier Data

Western blot - Anti-NOD1 antibody [EPR20833] - BSA and Azide free (AB238676)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

NOD1 is an alternative name for CARD4.

The 100-kDa band is the specific NOD1 band, other bands are unspecific.

The WT and NOD1 KO BMDMs lysates were kindly provided by our collaborator Dr. Gang Liu. Tsinghua University.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215726).

All lanes:

Western blot - Anti-NOD1 antibody [EPR20833] (<a href='/en-us/products/primary-antibodies/nod1-antibody-epr20833-ab215726'>ab215726</a>) at 1/1000 dilution

Lane 1:

Wild-type BMDM (mouse bone marrow-derived macrophages), whole cell lysate at 20 µg

Lane 2:

NOD1/CARD4 knockout BMDM, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 107 kDa

Observed band size: 100 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NOD1 antibody [EPR20833] - BSA and Azide free (AB238676)

This data was developed using ab215726, the same antibody clone in a different buffer formulation.

The 100-kDa band represents NOD1 monomer and the band above 250 kDa is the aggregates. Other bands are unspecific.

This antibody detects a strong BOI (band of interest) in mouse lung and mouse spleen, but a weak BOI in mouse heart which also expresses NOD1 (PMID : 25786158, PMID : 28878001, PMID : 23028889).

Loading control : Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NOD1 antibody [EPR20833] (<a href='/en-us/products/primary-antibodies/nod1-antibody-epr20833-ab215726'>ab215726</a>) at 1/1000 dilution

Lane 1:

Mouse lung lysate boiled before loading at 20 µg

Lane 2:

Mouse lung lysate not boiled before loading at 20 µg

Lane 3:

Mouse spleen lysate boiled before loading at 20 µg

Lane 4:

Mouse spleen lysate not boiled before loading at 20 µg

Lane 5:

Mouse heart lysate boiled before loading at 20 µg

Lane 6:

Mouse heart lysate not boiled before loading at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 107 kDa

Observed band size: 100 kDa

false

Exposure time: 3min

Unconjugated

Anti-NOD1 antibody [EPR20833]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20833

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab238676 is the carrier-free version of ab215726.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOD1 also known as nucleotide-binding oligomerization domain-containing protein 1 is a protein that plays a role as a recognition receptor within the immune system. It has an approximate mass of 103 kDa and is expressed in various tissues including the gastrointestinal tract and epithelial cells. NOD1 functions by detecting specific molecules from bacterial cell walls specifically diaminopimelic acid leading to the activation of immune responses. Through its interaction with bacterial peptidoglycans NOD1 plays an important function in the innate immune defense against pathogens.

Biological function summary

NOD1 is involved in sensing bacterial infections and initiating immune responses. It participates as part of the NOD-like receptor (NLR) family contributing significantly to inflammatory signal transduction. Upon recognition of bacterial components NOD1 triggers signaling cascades that result in the activation of NF-κB leading to the production of pro-inflammatory cytokines. By orchestrating these molecular events NOD1 plays a role in maintaining homeostasis and responding to microbial threats which influences various immune-related processes.

Pathways

NOD1 is integral to the immune signaling pathway involving inflammation and cellular defense. It is associated with the NF-κB signaling pathway and MAPK pathway important in mounting an effective immune response. NOD1 interaction with RIPK2 a kinase protein facilitates downstream activation of these pathways enhancing cytokine production and strengthening the body's defense mechanisms. These pathways enable NOD1 to communicate intracellularly and coordinate responses essential for pathogen clearance.

NOD1 has associations with inflammatory bowel disease (IBD) and asthma. Variants or dysregulations of NOD1 contribute to the susceptibility and pathology of these conditions. Within these disorders NOD1 activity can influence other proteins involved in inflammatory processes such as RIPK2 which acts synergistically to mediate immune responses. Understanding NOD1’s connection to these diseases may lead to therapeutic strategies targeting its signaling cascades to mitigate inflammation and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses (PubMed : 12796777, PubMed : 21715553). Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria (PubMed : 12796777, PubMed : 16211083). Preferentially binds iE-DAP in tetrapeptide-containing muropeptides (MurNAc-TetraDAP or TetraDAP) (PubMed : 16211083). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2 (By similarity). Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling (By similarity). This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (By similarity). Also acts as a regulator of antiviral response elicited by dsRNA and the expression of RLR pathway members by targeting IFIH1 and TRAF3 to modulate the formation of IFIH1-MAVS and TRAF3-MAVS complexes leading to increased transcription of type I IFNs (By similarity). Also acts as a regulator of autophagy via its interaction with ATG16L1, possibly by recruiting ATG16L1 at the site of bacterial entry (PubMed : 19898471). Besides recognizing pathogens, also involved in the endoplasmic reticulum stress response : acts by sensing and binding to the cytosolic metabolite sphingosine-1-phosphate generated in response to endoplasmic reticulum stress, initiating an inflammation process that leads to activation of the NF-kappa-B and MAP kinases signaling (PubMed : 27007849). In addition, plays a role in insulin trafficking in beta cells in a cell-autonomous manner (PubMed : 21715553, PubMed : 31201384). Mechanistically, upon recognizing cognate ligands, NOD1 and RIPK2 localize to insulin vesicles where they recruit RAB1A to direct insulin trafficking through the cytoplasm (PubMed : 31201384).

See full target information Nod1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOD1 antibody [EPR20833] - BSA and Azide free

Western blot - Anti-NOD1 antibody [EPR20833] - BSA and Azide free (AB238676)

Western blot - Anti-NOD1 antibody [EPR20833] - BSA and Azide free (AB238676)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise