AB81287

Anti-Nodal antibody [EP2058Y]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal NODAL antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 3 publications.

View Alternative Names

Nodal homolog, NODAL

1 Images

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Western blot - Anti-Nodal antibody [EP2058Y] (AB81287)

All lanes:

Western blot - Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution

All lanes:

Recombinant nodal protein at 10ng

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2058Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab81287 detects Pro-Nodal and Nodal. This antibody is unable to detect signal in endogenous samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "(Pro-Nodal) and 13 kDa (Nodal)." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nodal also known as NODAL protein is a growth factor belonging to the transforming growth factor beta (TGF-β) superfamily with a molecular mass of approximately 44 kDa. This protein acts mechanically by transmitting signals through receptors on the cell surface initiating intracellular responses critical for embryonic development. Nodal expression occurs mostly in early embryonic tissues including the primitive streak and visceral endoderm. In adults it shows limited expression yet you might find it in some cancer cells. Nodal antibodies can be used for research to study its expression patterns and roles in various contexts.

Biological function summary

Nodal plays an important role in processes such as mesoderm formation and left-right body axis specification. It is part of a signaling complex that includes co-factors like Cripto and receptor heteromers composed of Activin receptors and SMAD proteins. These interactions initiate cascades influencing cell fate decisions impacting diverse biological activities like cell proliferation and migration. Antinodal mechanisms act to modulate these signals ensuring proper gradient formation necessary for precise developmental outcomes.

Pathways

Nodal integrally fits within the TGF-β signaling pathway and the Wnt signaling pathway. It closely interacts with proteins such as Lefty and PITX2. The TGF-β pathway involves multiple SMAD proteins directing Nodal signals toward gene expression changes important for embryonic patterning. Meanwhile interactions with the Wnt pathway provide cross-talk opportunities that can affect a cell's destiny highlighting Nodal's broad regulatory capacity in embryogenesis.

Nodal is implicated in conditions like cancer and congenital abnormalities. In cancer aberrant Nodal expression connects with increased tumor aggressiveness and metastasis often through pathways involving proteins such as E-cadherin and N-cadherin which are important for cell adhesion and motility. In congenital disorders mutations or dysregulation in Nodal signaling can result in defects like holoprosencephaly often tied to the Hedgehog signaling pathway illustrating its critical developmental contributions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for mesoderm formation and axial patterning during embryonic development.

See full target information Nodal homolog

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 39:2855-2864 PubMed29693169

2018

Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Li,Junjie Chen,Xiao Wang,Mei He,Zhenyu Zhang,Ying Cen

Oncotarget 6:34071-86 PubMed26460952

2015

Effects of a novel Nodal-targeting monoclonal antibody in melanoma.

Applications

sELISA

Species

Human

Luigi Strizzi,Annamaria Sandomenico,Naira V Margaryan,Annalia Focà,Luca Sanguigno,Thomas M Bodenstine,Grace S Chandler,David W Reed,Alina Gilgur,Elisabeth A Seftor,Richard E B Seftor,Zhila Khalkhali-Ellis,Antonio Leonardi,Menotti Ruvo,Mary J C Hendrix

Journal of cellular physiology 225:390-3 PubMed20568225

2010

Cancer hallmarks in induced pluripotent cells: new insights.

Applications

Species

Human

Sergey Malchenko,Vasiliy Galat,Elisabeth A Seftor,Elio F Vanin,Fabricio F Costa,Richard E B Seftor,Marcelo B Soares,Mary J C Hendrix

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nodal antibody [EP2058Y]

Western blot - Anti-Nodal antibody [EP2058Y] (AB81287)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise