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AB242339

Anti-Nodal antibody [EPR20723-16] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal NODAL antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Nodal homolog, NODAL

1 Images
Sandwich ELISA - Anti-Nodal antibody [EPR20723-16] - BSA and Azide free (Capture) (AB242339)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Nodal antibody [EPR20723-16] - BSA and Azide free (Capture) (AB242339)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab256391).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20723-16

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab242339 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Nodal. The recommended pair for sandwich ELISA is:
Capture: ab242339, Human Nodal Capture Antibody (unconjugated)
Detector: ab242340, Human Nodal Detector Antibody (unconjugated)
The reference range value is 0.783 - 50 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nodal also known as NODAL protein is a growth factor belonging to the transforming growth factor beta (TGF-β) superfamily with a molecular mass of approximately 44 kDa. This protein acts mechanically by transmitting signals through receptors on the cell surface initiating intracellular responses critical for embryonic development. Nodal expression occurs mostly in early embryonic tissues including the primitive streak and visceral endoderm. In adults it shows limited expression yet you might find it in some cancer cells. Nodal antibodies can be used for research to study its expression patterns and roles in various contexts.
Biological function summary

Nodal plays an important role in processes such as mesoderm formation and left-right body axis specification. It is part of a signaling complex that includes co-factors like Cripto and receptor heteromers composed of Activin receptors and SMAD proteins. These interactions initiate cascades influencing cell fate decisions impacting diverse biological activities like cell proliferation and migration. Antinodal mechanisms act to modulate these signals ensuring proper gradient formation necessary for precise developmental outcomes.

Pathways

Nodal integrally fits within the TGF-β signaling pathway and the Wnt signaling pathway. It closely interacts with proteins such as Lefty and PITX2. The TGF-β pathway involves multiple SMAD proteins directing Nodal signals toward gene expression changes important for embryonic patterning. Meanwhile interactions with the Wnt pathway provide cross-talk opportunities that can affect a cell's destiny highlighting Nodal's broad regulatory capacity in embryogenesis.

Nodal is implicated in conditions like cancer and congenital abnormalities. In cancer aberrant Nodal expression connects with increased tumor aggressiveness and metastasis often through pathways involving proteins such as E-cadherin and N-cadherin which are important for cell adhesion and motility. In congenital disorders mutations or dysregulation in Nodal signaling can result in defects like holoprosencephaly often tied to the Hedgehog signaling pathway illustrating its critical developmental contributions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for mesoderm formation and axial patterning during embryonic development.
See full target information Nodal homolog
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com