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AB151416

Anti-Noelin antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal Noelin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OLFM1 aa 400 to C-terminus.

View Alternative Names

NOE1, NOEL1, OLFM1, Noelin, Neuronal olfactomedin-related ER localized protein, Olfactomedin-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (AB151416)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (AB151416)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Noelin with ab151416 at 1/500 dilution.
Lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (AB151416)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (AB151416)

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labeling Noelin with ab151416 at 1/100 dilution.

Western blot - Anti-Noelin antibody - C-terminal (AB151416)
  • WB

Unknown

Western blot - Anti-Noelin antibody - C-terminal (AB151416)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Noelin antibody - C-terminal (ab151416) at 1/500 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 55 kDa

false

Western blot - Anti-Noelin antibody - C-terminal (AB151416)
  • WB

Unknown

Western blot - Anti-Noelin antibody - C-terminal (AB151416)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Noelin antibody - C-terminal (ab151416) at 1/1000 dilution

All lanes:

mouse brain tissue lysate at 50 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human OLFM1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99784

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

The immunogen was raised from noelin isoform 1 precursor(NP_055094)
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Noelin also known as Olfactomedin 1 (OLFM1) is a secreted glycoprotein with a molecular mass around 55 kDa. It is expressed in the central nervous system particularly within the brain and retina and also in some peripheral tissues. The protein plays roles in synaptic function due to its ability to bind to components within the extracellular matrix.
Biological function summary

Noelin contributes to the stabilization of synaptic structures and regulation of neuronal network formation. It interacts with other olfactomedin domain-containing proteins and forms complexes to mediate its functions. These interactions help maintain synaptic plasticity which is important for learning and memory processes.

Pathways

The target takes part in neural development and function. It is involved in the Wnt signaling pathway important for cellular communication in brain development. Noelin also interacts with axon guidance molecules like netrins facilitating proper neuronal network formation. This places Noelin in important roles where fine-tuning synaptic connectivity is necessary.

Noelin has connections to neurological conditions such as schizophrenia and neurodegenerative diseases. Alterations in Noelin expression or function can disrupt synaptic connectivity potentially leading to these disorders. The protein interacts with other proteins like DISC1 in schizophrenia highlighting its importance in maintaining mental health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the regulation of axonal growth in the embryonic and adult central nervous system by inhibiting interactions between RTN4R and LINGO1. Inhibits RTN4R-mediated axon growth cone collapse (By similarity). May play an important role in regulating the production of neural crest cells by the neural tube (By similarity). May be required for normal responses to olfactory stimuli (By similarity).
See full target information OLFM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0130888 PubMed26121352

2015

Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon E Hill,Rebecca K Donegan,Elaine Nguyen,Tanay M Desai,Raquel L Lieberman
View all publications
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Product promise

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