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AB248637

Anti-Noelin antibody [EPR6439(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Noelin antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

NOE1, NOEL1, OLFM1, Noelin, Neuronal olfactomedin-related ER localized protein, Olfactomedin-1

1 Images
Western blot - Anti-Noelin antibody [EPR6439(2)] - BSA and Azide free (AB248637)
  • WB

Unknown

Western blot - Anti-Noelin antibody [EPR6439(2)] - BSA and Azide free (AB248637)

This data was developed using ab133746, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Noelin antibody [EPR6439(2)] (<a href='/en-us/products/primary-antibodies/noelin-antibody-epr64392-ab133746'>ab133746</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate

Lane 2:

Human hypothalamus tissue lysate

Lane 3:

Human plasma tissue lysate

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6439(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248637 is the carrier-free version of ab133746.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Noelin also known as Olfactomedin 1 (OLFM1) is a secreted glycoprotein with a molecular mass around 55 kDa. It is expressed in the central nervous system particularly within the brain and retina and also in some peripheral tissues. The protein plays roles in synaptic function due to its ability to bind to components within the extracellular matrix.
Biological function summary

Noelin contributes to the stabilization of synaptic structures and regulation of neuronal network formation. It interacts with other olfactomedin domain-containing proteins and forms complexes to mediate its functions. These interactions help maintain synaptic plasticity which is important for learning and memory processes.

Pathways

The target takes part in neural development and function. It is involved in the Wnt signaling pathway important for cellular communication in brain development. Noelin also interacts with axon guidance molecules like netrins facilitating proper neuronal network formation. This places Noelin in important roles where fine-tuning synaptic connectivity is necessary.

Noelin has connections to neurological conditions such as schizophrenia and neurodegenerative diseases. Alterations in Noelin expression or function can disrupt synaptic connectivity potentially leading to these disorders. The protein interacts with other proteins like DISC1 in schizophrenia highlighting its importance in maintaining mental health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the regulation of axonal growth in the embryonic and adult central nervous system by inhibiting interactions between RTN4R and LINGO1. Inhibits RTN4R-mediated axon growth cone collapse (By similarity). May play an important role in regulating the production of neural crest cells by the neural tube (By similarity). May be required for normal responses to olfactory stimuli (By similarity).
See full target information OLFM1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com