AB181161

Anti-NOL10 antibody [EPR14073-9]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal NOL10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Nucleolar protein 10, NOL10

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (AB181161)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (AB181161)

Immunocytochemical analysis of Hela cells fixed in acetone at -20°C labeling NOL10 using ab181161 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green) and counterstained with DAPI (blue)

Supplier Data

Western blot - Anti-NOL10 antibody [EPR14073-9] (AB181161)

All lanes:

Western blot - Anti-NOL10 antibody [EPR14073-9] (ab181161) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Huvec cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 80 kDa

false

Carrier free

Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14073-9

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL10 also known as nucleolar protein 10 functions primarily in the nucleolus of the cell playing roles in ribosome biogenesis. NOL10 weighs approximately 73 kDa and is present in different human tissues most expressed in spleen and bone marrow. This protein participates in important cellular processes by interacting with ribosomal RNA precursors during ribosome assembly. Its positioning in the nucleolus hints at a focus on ribosome production.

Biological function summary

NOL10 contributes to ribosomal RNA synthesis and processing. It associates with ribosomal RNA processing complexes ensuring mature ribosomal RNA formation. NOL10 works closely with other nucleolar proteins forming part of a larger pre-ribosomal assembly essential for protein synthesis. By doing this NOL10 indirectly supports translation and cell growth.

Pathways

NOL10 is involved in the ribosome biogenesis and RNA processing pathways. It connects with pathways such as the ribosome synthesis pathway interacting with proteins like fibrillarin to promote production of functional ribosomes. It ensures efficient processing within these pathways linking the initial steps of ribosome creation to later stages of protein synthesis.

NOL10's role in ribosome biogenesis links it to conditions like dyskeratosis congenita and certain cancers. Abnormal expression or mutations in NOL10 can result in disrupted ribosomal function contributing to disease progression. It has a connection with proteins such as dyskerin in dyskeratosis congenita highlighting its role in maintaining normal cellular and tissue function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information NOL10

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:9 PubMed36599853

2023

PQBP5/NOL10 maintains and anchors the nucleolus under physiological and osmotic stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocen Jin,Hikari Tanaka,Meihua Jin,Kyota Fujita,Hidenori Homma,Maiko Inotsume,Huang Yong,Kenichi Umeda,Noriyuki Kodera,Toshio Ando,Hitoshi Okazawa

iScience 25:104289 PubMed35573189

2022

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Iwasaki,Yuka Kawahara,Chikako Okubo,Tatsuya Yamakawa,Michiko Nakamura,Tsuyoshi Tabata,Yohei Nishi,Megumi Narita,Akira Ohta,Hirohide Saito,Takuya Yamamoto,Masato Nakagawa,Shinya Yamanaka,Kazutoshi Takahashi

Frontiers in oncology 10:541479 PubMed33102213

2020

Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Hu,Mingyang Bao,Jiacheng Huang,Lin Zhou,Shusen Zheng

Nucleic acids research 44:9803-9820 PubMed27599843

2016

Human AATF/Che-1 forms a nucleolar protein complex with NGDN and NOL10 required for 40S ribosomal subunit synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Bammert,Stefanie Jonas,Rosemarie Ungricht,Ulrike Kutay

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOL10 antibody [EPR14073-9]

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] (AB181161)

Western blot - Anti-NOL10 antibody [EPR14073-9] (AB181161)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise