AB181996

Anti-NOL11 antibody [EPR14074-30]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NOL11 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

L14, NOL11, Nucleolar protein 11

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunofluorescent analysis of -20°C acetone-fixed Hela cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain : Dapi (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain : Dapi (blue).

Supplier Data

Western blot - Anti-NOL11 antibody [EPR14074-30] (AB181996)

All lanes:

Western blot - Anti-NOL11 antibody [EPR14074-30] (ab181996) at 1/5000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 146 kDa,81 kDa,91 kDa

Observed band size: 120 kDa

false

Carrier free

Anti-NOL11 antibody [EPR14074-30] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14074-30

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL11 also known as Nucleolar Protein 11 plays a mechanical role in ribosome biogenesis. This protein is 77 kDa in mass and shows expression largely in the nucleolus of the cell. It participates in the proper processing of the 47S pre-ribosomal RNA (rRNA) into the mature 18S rRNA an essential step in the formation of functional ribosomes. NOL11 interacts with elements of the small-subunit (SSU) processome indicating its involvement in early stages of ribosome assembly.

Biological function summary

NOL11 contributes to cellular proliferation by ensuring efficient ribosome synthesis. It acts as a component of the SSU processome complex which is a large ribonucleoprotein complex essential for the maturation of pre-rRNA. The protein NOL11 serves as a critical factor required for the synthesis of rRNA a process that directly affects the production of ribosomes and subsequently protein synthesis influencing cell growth and division.

Pathways

The protein functions within two major biological pathways: ribosome biogenesis and the cell cycle. It has a central role in ribosome biogenesis impacting the processing of pre-rRNA and connecting with other rRNA processing factors like Utp21. NOL11 also influences the cell cycle by indirectly affecting cell growth through its impact on ribosome production tying in with growth control mechanisms that involve proteins like p53.

NOL11 shows associations with conditions such as North American Indian childhood cirrhosis and Diamond-Blackfan anemia. These connections arise due to its pivotal function in ribosome biogenesis; any impairment can disrupt cellular protein synthesis leading to such disorders. NOL11 interacts with proteins involved in these conditions like those in the Fanconi anemia related pathways which further establishes its role in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ribosome biogenesis factor. May be required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b.

See full target information NOL11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e02838 PubMed40548932

2025

CD74 Blockade Disrupts Endothelial Migrasome Signaling to Prevent Inflammatory Macrophage Differentiation and Inhibit Atherosclerotic Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kangnan Zhang,Jiong Chen,Zhenhua Zhu,Hong Hu,Qinghui Zhang,Rongrong Jia,Na Wang,Shihao Xiang,Yong Zhou,Yuehong Wang,Ling Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOL11 antibody [EPR14074-30]

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Western blot - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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