JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250144

Anti-NOL11 antibody [EPR14074] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal NOL11 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

L14, NOL11, Nucleolar protein 11

1 Images
Western blot - Anti-NOL11 antibody [EPR14074] - BSA and Azide free (AB250144)
  • WB

Supplier Data

Western blot - Anti-NOL11 antibody [EPR14074] - BSA and Azide free (AB250144)

This data was developed using ab180132, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NOL11 antibody [EPR14074] (<a href='/en-us/products/primary-antibodies/nol11-antibody-epr14074-ab180132'>ab180132</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14074

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250144 is the carrier-free version of ab180132.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL11 also known as Nucleolar Protein 11 plays a mechanical role in ribosome biogenesis. This protein is 77 kDa in mass and shows expression largely in the nucleolus of the cell. It participates in the proper processing of the 47S pre-ribosomal RNA (rRNA) into the mature 18S rRNA an essential step in the formation of functional ribosomes. NOL11 interacts with elements of the small-subunit (SSU) processome indicating its involvement in early stages of ribosome assembly.
Biological function summary

NOL11 contributes to cellular proliferation by ensuring efficient ribosome synthesis. It acts as a component of the SSU processome complex which is a large ribonucleoprotein complex essential for the maturation of pre-rRNA. The protein NOL11 serves as a critical factor required for the synthesis of rRNA a process that directly affects the production of ribosomes and subsequently protein synthesis influencing cell growth and division.

Pathways

The protein functions within two major biological pathways: ribosome biogenesis and the cell cycle. It has a central role in ribosome biogenesis impacting the processing of pre-rRNA and connecting with other rRNA processing factors like Utp21. NOL11 also influences the cell cycle by indirectly affecting cell growth through its impact on ribosome production tying in with growth control mechanisms that involve proteins like p53.

NOL11 shows associations with conditions such as North American Indian childhood cirrhosis and Diamond-Blackfan anemia. These connections arise due to its pivotal function in ribosome biogenesis; any impairment can disrupt cellular protein synthesis leading to such disorders. NOL11 interacts with proteins involved in these conditions like those in the Fanconi anemia related pathways which further establishes its role in disease pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ribosome biogenesis factor. May be required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b.
See full target information NOL11
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB171943

Anti-SGSM1/RUTBC2 antibody [EPR11563]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

  • 0
  • 0
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Primary Antibodies

AB45139

Anti-Vitronectin/S-Protein antibody [EP873Y]

RabMAb|Recombinant

Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (AB45139)

  • 0
  • 0
Primary Antibodies

AB220161

Anti-YTHDF3 antibody [EPR21912-3]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [EPR21912-3] (AB220161)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com