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Rabbit Recombinant Monoclonal NOL3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304), expandable thumbnail
  • Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304), expandable thumbnail
  • Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Tested
Expected
Rat
Expected
Expected
Tested
Tested
Expected

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Isoform 1May be involved in RNA splicing.Isoform 2Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:15004034). Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (PubMed:15509781). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (By similarity). Inhibits too myoblast differentiation through caspase inhibition (By similarity).

Alternative names

Nucleolar protein 3, Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Nucleolar protein of 30 kDa, Myp, Nop30, NOL3, ARC, NOP

Recommended products

Rabbit Recombinant Monoclonal NOL3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25182-11

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab288304 is the carrier-free version of Anti-NOL3 antibody [EPR25182-11] ab288295.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nucleolar protein 3 (NOL3) also known as ARC (apoptosis repressor with caspase recruitment domain) is a multifunctional protein involved in apoptosis regulation and cellular stress responses. It has a molecular mass of approximately 26 kDa. NOL3 is expressed in various tissues but it displays high levels in the heart skeletal muscles and neurons. Its expression suggests a protective role in these tissues where it interacts with various cellular components to prevent apoptosis.

Biological function summary

NOL3 contributes significantly to cell survival and apoptosis inhibition by interacting with pro-apoptotic proteins to block their function. It acts as a safeguard against external stressors that can trigger cell death. As part of its role in apoptosis regulation NOL3 engages in complex formation with other proteins to modulate apoptotic pathways. For instance it binds directly to caspases preventing their activation and further propagation of cell death signals.

Pathways

NOL3 plays a critical role in the apoptosis and stress response pathways. It participates in the intrinsic apoptosis pathway by inhibiting the activation of key apoptotic enzymes while also interacting with proteins such as Bcl-2 and Bax to further regulate cell survival. The involvement of NOL3 in stress response pathways highlights its function in promoting cellular resilience in challenging conditions often functioning together with heat shock proteins that assist in protein folding and stress recovery.

Associated diseases and disorders

NOL3 has been implicated in neurodegenerative diseases and cardiac dysfunction. In particular its dysregulation is associated with conditions like Alzheimer's disease where it might influence neural cell survival. Furthermore NOL3 interacts with amyloid precursor protein (APP) which has a role in Alzheimer's pathology. In the context of cardiac diseases altered NOL3 expression may influence cardiac hypertrophy and heart failure potentially interacting with proteins such as caspase-8 in these processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with Anti-NOL3 antibody [EPR25182-11] ab288295 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer: 5% NFDM/TBST.

    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 14645204, 20026055, 10590251).

    Low expression: Mouse liver (PMID: 14645204)

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-NOL3 antibody [EPR25182-11] (Anti-NOL3 antibody [EPR25182-11] ab288295) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat heart tissue lysate at 20 µg

    Lane 5: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa

    Observed band size: 27 kDa

  • Flow Cytometry (Intracellular) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/5000 dilution (0.01ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Daudi (PMID: HPA database).

  • Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in Neuro-2a cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 15848180).

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-NOL3 antibody [EPR25182-11] (Anti-NOL3 antibody [EPR25182-11] ab288295) at 1/1000 dilution

    Lane 1: Human heart tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 24 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Western blot - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 17142452).

    Negative control: Daudi (PMID: HPA database)

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-NOL3 antibody [EPR25182-11] (Anti-NOL3 antibody [EPR25182-11] ab288295) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa

    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with Anti-NOL3 antibody [EPR25182-11] ab288295 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    NOL3 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: Anti-NOL3 antibody [EPR25182-11] ab288295 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NOL3 antibody [EPR25182-11] ab288295 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    All lanes: Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] (Anti-NOL3 antibody [EPR25182-11] ab288295)

    Predicted band size: 24 kDa

    Observed band size: 27 kDa

  • Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    NOL3 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: Anti-NOL3 antibody [EPR25182-11] ab288295 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NOL3 antibody [EPR25182-11] ab288295 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-NOL3 antibody [EPR25182-11] (Anti-NOL3 antibody [EPR25182-11] ab288295)

    Predicted band size: 24 kDa

    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle. The section was incubated with Anti-NOL3 antibody [EPR25182-11] ab288295 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used. Negative control: No staining on mouse liver. The section was incubated with Anti-NOL3 antibody [EPR25182-11] ab288295 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (ab288304)

    This data was developed using Anti-NOL3 antibody [EPR25182-11] ab288295, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 cells labelling NOL3 with Anti-NOL3 antibody [EPR25182-11] ab288295 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in MCF7 cell line. Negative control: Daudi. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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