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AB242201

Anti-NOL5A antibody [CL2603]

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(1 Publication)

Mouse Monoclonal NOL5A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOP56 aa 450-550.

View Alternative Names

NOL5A, NOP56, Nucleolar protein 56, Nucleolar protein 5A

13 Images
Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human liver tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human kidney tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human cervix tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of U-251 MG (Human brain glioma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)
  • WB

Supplier Data

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

Loading control : anti-GAPDH.

All lanes:

Western blot - Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/mL

Lane 1:

Non-transfected U-251 MG (Human brain glioma cell line) cell lysate

Lane 2:

siRNA#1 transfected U-251 MG cell lysate

Lane 3:

siRNA#2 transfected U-251 MG cell lysate

Predicted band size: 66 kDa

false

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)
  • WB

Supplier Data

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

All lanes:

Western blot - Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/mL

All lanes:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 66 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2603

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NOP56 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

O00567

Epitope

Binds to an epitope located within the peptide sequence FSKEELMSSDLEETA as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL5A also known as Nucleolar Protein 5A or hNop56 functions as a nucleolar protein involved in ribosome biogenesis. It is characterized by a mass of approximately 63 kDa. NOL5A exhibits high expression within the nucleolus of cells where it participates in the assembly of ribosomal subunits. Specifically NOL5A plays a role in the modification and maturation of pre-ribosomal RNA. Its expression is observed in rapidly dividing cells highlighting its importance in cellular growth and proliferation.
Biological function summary

Ribosome biogenesis depends on several proteins and NOL5A serves as a core component in the small nucleolar ribonucleoprotein (snoRNP) complex. This complex is responsible for the processing and chemical modification of small nucleolar RNAs (snoRNAs) which are essential for the production of functional ribosomes. NOL5A helps facilitate the cleavage and assembly steps necessary for pre-rRNA to mature into functional rRNAn important for effective protein synthesis within the cell. Its activity is important to maintaining proper ribosome function ensuring efficient protein translation.

Pathways

NOL5A acts within the rRNA processing pathway and is also linked to the mTOR signaling pathway. In the rRNA processing pathway NOL5A's role in the nucleoplasm involves cooperation with other nucleolar proteins like fibrillarin and Nop58 to orchestrate rRNA processing. In the mTOR pathway the regulation of ribosome production connects NOL5A to protein synthesis pathways impacting cell growth and proliferation. mTOR signaling influences many cellular processes and NOL5A’s function in ribosome biogenesis links it to these critical growth and survival pathways.

NOL5A has connections with cancer and ribosomopathy. Altered expression of NOL5A has implications in various cancers as its role in ribosome production can drive aberrant cell proliferation. Additionally proper function of NOL5A is essential in preventing ribosomopathies disorders characterized by defects in ribosome biogenesis. These disorders often result in bone marrow failure or craniofacial abnormalities. Studies investigating NOL5A's interactions with proteins such as NPM1 show its involvement in nucleolar stress responses which can influence cancer progression and response to therapy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs (PubMed : 12777385, PubMed : 15574333). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs) (PubMed : 12777385, PubMed : 39570315).
See full target information NOP56
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 23:84 PubMed35126726

2022

Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Mao,Jun Wang,Fen Luo
View all publications
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