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AB228836

Anti-NOL6 antibody

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(1 Publication)

Rabbit Polyclonal NOL6 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Nucleolar protein 6.

View Alternative Names

Nucleolar protein 6, Nucleolar RNA-associated protein, Nrap, NOL6

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NOL6 antibody (AB228836)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL6 antibody (AB228836)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL6 (green) using ab228836 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Counterstain : Hoechst 33342 (blue) and alpha tubulin filaments (red).

Western blot - Anti-NOL6 antibody (AB228836)
  • WB

Supplier Data

Western blot - Anti-NOL6 antibody (AB228836)

12% SDS-PAGE

All lanes:

Western blot - Anti-NOL6 antibody (ab228836) at 1/1000 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Predicted band size: 127 kDa

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Western blot - Anti-NOL6 antibody (AB228836)
  • WB

Supplier Data

Western blot - Anti-NOL6 antibody (AB228836)

5% SDS-PAGE

All lanes:

Western blot - Anti-NOL6 antibody (ab228836) at 1/500 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 127 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Nucleolar protein 6. The exact immunogen used to generate this antibody is proprietary information.

Q9H6R4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL6 also known as nucleolar protein 6 is a nucleolar RNA-associated protein with a mass of roughly 117 kDa. It localizes mainly to the nucleolus and shows high expression in actively dividing cells reflecting its role in ribosomal RNA (rRNA) processing. The NOL6 protein interacts with rRNA during ribosome biogenesis and its presence is notable in developing and differentiating tissues pointing to its involvement in fundamental cell growth and proliferation mechanisms.
Biological function summary

NOL6 participates in ribosomal assembly by interacting with pre-ribosomal particles. It becomes part of the larger ribonucleoprotein complex important for the maturation of the 60S ribosomal subunit. NOL6 ensures the accurate cleavage and modification of precursor rRNA which is essential for functional ribosome construction. This contribution influences protein synthesis rates within the cell impacting overall cellular metabolism and responsiveness to growth signals.

Pathways

Ribosome biogenesis and RNA processing in the nucleolus engage NOL6 extensively. It closely interacts with the rRNA processing pathway and is functionally connected to other nucleolar proteins such as fibrillarin and NOP56. These proteins coordinate for effective ribosome production ensuring the cell's protein synthesis machinery runs smoothly. Through these pathways NOL6 assists in maintaining cellular growth and response to environmental change.

NOL6 has implications in cancer and ribosomopathies conditions tied to defective ribosome biogenesis. Aberrant expression or mutation of NOL6 has been observed in various cancers associating it with dysregulated cell growth. It can also connect to oncogenes like c-Myc which modulates ribosome biogenesis and cell proliferation. Understanding these relationships helps identify potential diagnostic and therapeutic targets providing insights into combating these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
See full target information Nucleolar protein 6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht) 45:355-365 PubMed35579750

2022

Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated mA demethylation of EIF4EBP1 and MLST8 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Xiulan Su
View all publications
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Product promise

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