Rabbit Polyclonal NOL6 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Nucleolar protein 6.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Nucleolar protein 6. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H6R4
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Target data
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Alternative names
Nucleolar protein 6, Nucleolar RNA-associated protein, Nrap, NOL6
Recommended products
Rabbit Polyclonal NOL6 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Nucleolar protein 6.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Nucleolar protein 6. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H6R4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOL6 also known as nucleolar protein 6 is a nucleolar RNA-associated protein with a mass of roughly 117 kDa. It localizes mainly to the nucleolus and shows high expression in actively dividing cells reflecting its role in ribosomal RNA (rRNA) processing. The NOL6 protein interacts with rRNA during ribosome biogenesis and its presence is notable in developing and differentiating tissues pointing to its involvement in fundamental cell growth and proliferation mechanisms.
- Biological function summary
NOL6 participates in ribosomal assembly by interacting with pre-ribosomal particles. It becomes part of the larger ribonucleoprotein complex important for the maturation of the 60S ribosomal subunit. NOL6 ensures the accurate cleavage and modification of precursor rRNA which is essential for functional ribosome construction. This contribution influences protein synthesis rates within the cell impacting overall cellular metabolism and responsiveness to growth signals.
- Pathways
Ribosome biogenesis and RNA processing in the nucleolus engage NOL6 extensively. It closely interacts with the rRNA processing pathway and is functionally connected to other nucleolar proteins such as fibrillarin and NOP56. These proteins coordinate for effective ribosome production ensuring the cell's protein synthesis machinery runs smoothly. Through these pathways NOL6 assists in maintaining cellular growth and response to environmental change.
- Associated diseases and disorders
NOL6 has implications in cancer and ribosomopathies conditions tied to defective ribosome biogenesis. Aberrant expression or mutation of NOL6 has been observed in various cancers associating it with dysregulated cell growth. It can also connect to oncogenes like c-Myc which modulates ribosome biogenesis and cell proliferation. Understanding these relationships helps identify potential diagnostic and therapeutic targets providing insights into combating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-NOL6 antibody (ab228836)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL6 (green) using ab228836 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Counterstain: Hoechst 33342 (blue) and alpha tubulin filaments (red).
Western blot - Anti-NOL6 antibody (ab228836)
5% SDS-PAGE
All lanes: Western blot - Anti-NOL6 antibody (ab228836) at 1/500 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
Western blot - Anti-NOL6 antibody (ab228836)
12% SDS-PAGE
All lanes: Western blot - Anti-NOL6 antibody (ab228836) at 1/1000 dilution
All lanes: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
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