AB140597

Anti-NOL9 antibody [EPR9578]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal NOL9 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Polynucleotide 5'-hydroxyl-kinase NOL9, Nucleolar protein 9, NOL9

1 Images

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Western blot - Anti-NOL9 antibody [EPR9578] (AB140597)

All lanes:

Western blot - Anti-NOL9 antibody [EPR9578] (ab140597) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

Carrier free

Anti-NOL9 antibody [EPR9578] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9578

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL9 also known as Nucleolar Protein 9 is a protein encoded by the NOL9 gene. It has a molecular mass of approximately 150 kDa. This protein mainly localizes in the nucleolus a sub-region of the nucleus where it is highly expressed. NOL9 functions as a polynucleotide 5'-kinase which means it is involved in adding phosphate groups to the 5' end of RNA molecules. This activity plays an important role in processes such as RNA maturation.

Biological function summary

NOL9 participates in ribosome biogenesis which is the process of making ribosomes. It plays an important part in the processing and modification of precursor ribosomal RNA (pre-rRNA) into mature ribosomal RNA (rRNA) a task that is key for proper ribosome functionality. NOL9 functions within a multiprotein complex engaging with other nucleolar proteins to achieve efficient ribosomal RNA processing. The proper assembly and function of this complex ensure correct ribosome production a vital step for protein synthesis within the cell.

Pathways

NOL9 is involved in the rRNA processing pathway and ribosome assembly. It collaborates with other important proteins like fibrillarin and nucleolin to guide the transformation of pre-rRNA into functional rRNA components. This transformation is part of the ribosome biogenesis pathway which supports cellular growth and protein synthesis. The efficient functioning of NOL9 in these pathways helps maintain cellular homeostasis and protein production.

NOL9 has been related to potential implications in cancer and genetic ribosomopathy. Its role in ribosome biogenesis links it to cancer as abnormal ribosome production can lead to uncontrolled cell division and growth. NOL9's interaction with proteins like nucleophosmin an oncogenic protein suggests a possible involvement in tumor progression. Additionally mutations or dysfunctions in NOL9 may contribute to ribosomopathy disorders linked to errors in ribosome function which includes conditions like Diamond-Blackfan Anemia.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of single-stranded and double-stranded RNA and DNA substrates (PubMed : 21063389). Involved in rRNA processing and its kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form (PubMed : 21063389). Required for the efficient pre-rRNA processing of internal transcribed spacer 2 (ITS2) (PubMed : 21063389). Associates with LAS1L to form an ITS2 pre-rRNA endonuclease-kinase complex and is responsible for the transport of this complex into the nucleolus (PubMed : 31288032).

See full target information Polynucleotide 5'-hydroxyl-kinase NOL9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 55:e51885 PubMed40538191

2025

NKG7 is a Stable Marker of Cytotoxicity Across Immune Contexts and Within the Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Turiello,Susanna S Ng,Elisabeth Tan,Gemma van der Voort,Nazhifah Salim,Michelle C R Yong,Malika Khassenova,Johannes Oldenburg,Heiko Rühl,Jan Hasenauer,Laura Surace,Marieta Toma,Tobias Bald,Michael Hölzel,Dillon Corvino

Cell death & disease 16:100 PubMed39955289

2025

Nucleolar NOL9 regulated by DNA methylation promotes hepatocellular carcinoma growth through activation of Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyao Chen,Xin Song,Xingrong Zheng,Tinglin Qian,Boxiang Zhang,Lina Wu,Qinghai Lian,Jia Chen,Qiumin Luo,Wenxiong Xu,Liang Peng,Chan Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOL9 antibody [EPR9578]

Western blot - Anti-NOL9 antibody [EPR9578] (AB140597)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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