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AB248893

Anti-NOL9 antibody [EPR9578] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NOL9 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Polynucleotide 5'-hydroxyl-kinase NOL9, Nucleolar protein 9, NOL9

1 Images
Western blot - Anti-NOL9 antibody [EPR9578] - BSA and Azide free (AB248893)
  • WB

Unknown

Western blot - Anti-NOL9 antibody [EPR9578] - BSA and Azide free (AB248893)

This data was developed using ab140597, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NOL9 antibody [EPR9578] (<a href='/en-us/products/primary-antibodies/nol9-antibody-epr9578-ab140597'>ab140597</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9578

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248893 is the carrier-free version of ab140597.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOL9 also known as Nucleolar Protein 9 is a protein encoded by the NOL9 gene. It has a molecular mass of approximately 150 kDa. This protein mainly localizes in the nucleolus a sub-region of the nucleus where it is highly expressed. NOL9 functions as a polynucleotide 5'-kinase which means it is involved in adding phosphate groups to the 5' end of RNA molecules. This activity plays an important role in processes such as RNA maturation.
Biological function summary

NOL9 participates in ribosome biogenesis which is the process of making ribosomes. It plays an important part in the processing and modification of precursor ribosomal RNA (pre-rRNA) into mature ribosomal RNA (rRNA) a task that is key for proper ribosome functionality. NOL9 functions within a multiprotein complex engaging with other nucleolar proteins to achieve efficient ribosomal RNA processing. The proper assembly and function of this complex ensure correct ribosome production a vital step for protein synthesis within the cell.

Pathways

NOL9 is involved in the rRNA processing pathway and ribosome assembly. It collaborates with other important proteins like fibrillarin and nucleolin to guide the transformation of pre-rRNA into functional rRNA components. This transformation is part of the ribosome biogenesis pathway which supports cellular growth and protein synthesis. The efficient functioning of NOL9 in these pathways helps maintain cellular homeostasis and protein production.

NOL9 has been related to potential implications in cancer and genetic ribosomopathy. Its role in ribosome biogenesis links it to cancer as abnormal ribosome production can lead to uncontrolled cell division and growth. NOL9's interaction with proteins like nucleophosmin an oncogenic protein suggests a possible involvement in tumor progression. Additionally mutations or dysfunctions in NOL9 may contribute to ribosomopathy disorders linked to errors in ribosome function which includes conditions like Diamond-Blackfan Anemia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of single-stranded and double-stranded RNA and DNA substrates (PubMed : 21063389). Involved in rRNA processing and its kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form (PubMed : 21063389). Required for the efficient pre-rRNA processing of internal transcribed spacer 2 (ITS2) (PubMed : 21063389). Associates with LAS1L to form an ITS2 pre-rRNA endonuclease-kinase complex and is responsible for the transport of this complex into the nucleolus (PubMed : 31288032).
See full target information Polynucleotide 5'-hydroxyl-kinase NOL9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

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