AB188617

Anti-NOLA1 antibody

Name: Anti-NOLA1 antibody (ab188617) | Abcam
Brand: Abcam Limited
SKU: AB188617
Rating: 3 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal NOLA1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GAR1 aa 50-100.

View Alternative Names

NOLA1, GAR1, H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-NOLA1 antibody (AB188617)

Immunocytochemistry analysis of Human cell line U-2 OS labeling NOLA1 with ab188617 at 2 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human GAR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NY12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOLA1 also known as NOP58 or nucleolar protein 58 is a core component of the box C/D small nucleolar ribonucleoprotein (snoRNP) complex important in pre-rRNA processing. The NOLA1 protein has a molecular mass of approximately 58 kDa. You will find it expressed mainly within the nucleolus of the cell where it participates in the ribosome biogenesis. The structural integrity of NOLA1 is necessary for its function allowing for interactions with other snoRNP components like fibrillarin and NOP56.

Biological function summary

NOLA1 participates in ribosomal RNA (rRNA) maturation and assembly making it indispensable for the synthesis of ribosomes. As a part of the box C/D snoRNP complex it orchestrates the methylation of rRNA by guiding and stabilizing methyltransferase activity provided by fibrillarin. This activity is essential for the modification of rRNA which is a requisite for proper ribosome assembly and function. The action of NOLA1 directly affects cellular protein synthesis by ensuring ribosomes are correctly assembled.

Pathways

The interactions and functions of NOLA1 mostly impact the ribosome biogenesis pathway. This pathway involves the transformation of pre-rRNA into mature rRNA a process critical for functional ribosome production. As a component of this pathway NOLA1 interacts with proteins like NOP56 and fibrillarin which jointly facilitate the methylation and processing of pre-rRNA. Involvement in these pathways underlines how NOLA1 supports cellular growth and protein synthesis efficiency.

Deregulation or mutations in NOLA1 have implicatory links to specific nucleolar stress conditions and various cancers. Disruptions in its function can lead to improper ribosome biogenesis affecting cancerous growth due to unregulated protein synthesis. NOLA1's role connects to proteins like p53 which monitors nucleolar stress and can trigger apoptotic pathways when stress is detected. This connection highlights its importance in maintaining cellular homeostasis and preventing pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

See full target information GAR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2023:9011232 PubMed36874625

2023

lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Song Guo,Bingtan Song,Lin Li,Hesheng Li,Tao Yang,Lianmeng Cao,Jian Wang

The EMBO journal 40:e106336 PubMed33595114

2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Raghunandan,Dan Geelen,Eva Majerova,Anabelle Decottignies

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOLA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (AB188617)

Immunocytochemistry - Anti-NOLA1 antibody (AB188617)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise