Rabbit Polyclonal NOLA1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GAR1 aa 50-100.
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Publications
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- The EMBO journal 40:e1063362021NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaya Raghunandan,Dan Geelen,Eva Majerova,Anabelle DecottigniesPubMed 33595114
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GAR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY12
Reactivity data
Select an application| IHC-P | ICC | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Alternative names
NOLA1, GAR1, H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1
Recommended products
Rabbit Polyclonal NOLA1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GAR1 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GAR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY12
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOLA1 also known as NOP58 or nucleolar protein 58 is a core component of the box C/D small nucleolar ribonucleoprotein (snoRNP) complex important in pre-rRNA processing. The NOLA1 protein has a molecular mass of approximately 58 kDa. You will find it expressed mainly within the nucleolus of the cell where it participates in the ribosome biogenesis. The structural integrity of NOLA1 is necessary for its function allowing for interactions with other snoRNP components like fibrillarin and NOP56.
- Biological function summary
NOLA1 participates in ribosomal RNA (rRNA) maturation and assembly making it indispensable for the synthesis of ribosomes. As a part of the box C/D snoRNP complex it orchestrates the methylation of rRNA by guiding and stabilizing methyltransferase activity provided by fibrillarin. This activity is essential for the modification of rRNA which is a requisite for proper ribosome assembly and function. The action of NOLA1 directly affects cellular protein synthesis by ensuring ribosomes are correctly assembled.
- Pathways
The interactions and functions of NOLA1 mostly impact the ribosome biogenesis pathway. This pathway involves the transformation of pre-rRNA into mature rRNA a process critical for functional ribosome production. As a component of this pathway NOLA1 interacts with proteins like NOP56 and fibrillarin which jointly facilitate the methylation and processing of pre-rRNA. Involvement in these pathways underlines how NOLA1 supports cellular growth and protein synthesis efficiency.
- Associated diseases and disorders
Deregulation or mutations in NOLA1 have implicatory links to specific nucleolar stress conditions and various cancers. Disruptions in its function can lead to improper ribosome biogenesis affecting cancerous growth due to unregulated protein synthesis. NOLA1's role connects to proteins like p53 which monitors nucleolar stress and can trigger apoptotic pathways when stress is detected. This connection highlights its importance in maintaining cellular homeostasis and preventing pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry - Anti-NOLA1 antibody (ab188617)
Immunocytochemistry analysis of Human cell line U-2 OS labeling NOLA1 with ab188617 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (ab188617)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (ab188617)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (ab188617)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (ab188617)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLA1 antibody (ab188617)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling NOLA1 with ab188617 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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