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AB204318

Anti-NOLC1 antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal NOLC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOLC1 aa 550-700.

View Alternative Names

KIAA0035, NS5ATP13, NOLC1, Nucleolar and coiled-body phosphoprotein 1, 140 kDa nucleolar phosphoprotein, Hepatitis C virus NS5A-transactivated protein 13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130, Nopp140, HCV NS5A-transactivated protein 13

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody (AB204318)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody (AB204318)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue, labeling NOLC1 with ab204318 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody (AB204318)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody (AB204318)

Immunofluorescent analysis of HEK 293 cells (PFA-fixed/Triton X-100 permeabilized) labeling NOLC1 with ab204318 at 4 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NOLC1 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q14978

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOLC1 which also goes by the alternative name nucleolar and coiled-body phosphoprotein 1 has an important role in cellular mechanics. This protein weighs around 76 kDa. NOLC1 localizes in the nucleolus which means it has a strong connection to ribosome biogenesis in the cell. You will often find NOLC1 expressed in various human tissues particularly those with high proliferative capacity.
Biological function summary

Nucleolus assembly and maintenance depend notably on pathways involving NOLC1. It acts as part of an essential multiprotein complex. NOLC1 interacts with other nucleolar proteins to maintain nucleolar structure and facilitate ribosomal RNA (rRNA) synthesis. Its activities support the structural and functional integrity needed for efficient ribosome production.

Pathways

Ribosome biogenesis forms the critical framework where NOLC1 operates. This protein interacts closely within the RNA polymerase I transcription machinery and rRNA processing pathways working alongside proteins like fibrillarin and UBF1. NOLC1's presence within these pathways helps in coordinating the transcriptional and structural elements necessary for ribosome assembly.

Aberrant NOLC1 function links to cancer and neurodegenerative diseases. Elevated NOLC1 levels sometimes correlate with increased tumor growth connecting it to malignant processes. Its interactions with proteins such as tumor protein p53 highlight its importance in regulating cell cycle and stress responses. Additionally disruptions in its nucleolar roles have potential associations with neurodegenerative disorders indicating its critical role beyond normal cellular functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed : 10567578, PubMed : 26399832). Required for neural crest specification : following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed : 26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed : 9016786). It has intrinsic GTPase and ATPase activities (PubMed : 9016786).
See full target information NOLC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:69 PubMed38460058

2024

DDOST is associated with tumor immunosuppressive microenvironment in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Mei,Liuliu Pan,Min Huang,Dandan Bao,Hui Gao,Danhan Wang
View all publications
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Product promise

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