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AB246325

Anti-NOLC1 antibody [EPR14896] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NOLC1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0035, NS5ATP13, NOLC1, Nucleolar and coiled-body phosphoprotein 1, 140 kDa nucleolar phosphoprotein, Hepatitis C virus NS5A-transactivated protein 13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130, Nopp140, HCV NS5A-transactivated protein 13

3 Images
Flow Cytometry (Intracellular) - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling NOLC1 with ab184550 at a 1/300 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184550).

Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)

Immunofluorescence staining of -20°C Acetone fixed K562 cells labeling NOLC1 using ab184550 at a 1/500 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution. Counterstained with Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184550).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] - BSA and Azide free (AB246325)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling NOLC1 at a 1/100 dilution, counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184550).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14896

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab246325 is the carrier-free version of ab184550.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOLC1 which also goes by the alternative name nucleolar and coiled-body phosphoprotein 1 has an important role in cellular mechanics. This protein weighs around 76 kDa. NOLC1 localizes in the nucleolus which means it has a strong connection to ribosome biogenesis in the cell. You will often find NOLC1 expressed in various human tissues particularly those with high proliferative capacity.
Biological function summary

Nucleolus assembly and maintenance depend notably on pathways involving NOLC1. It acts as part of an essential multiprotein complex. NOLC1 interacts with other nucleolar proteins to maintain nucleolar structure and facilitate ribosomal RNA (rRNA) synthesis. Its activities support the structural and functional integrity needed for efficient ribosome production.

Pathways

Ribosome biogenesis forms the critical framework where NOLC1 operates. This protein interacts closely within the RNA polymerase I transcription machinery and rRNA processing pathways working alongside proteins like fibrillarin and UBF1. NOLC1's presence within these pathways helps in coordinating the transcriptional and structural elements necessary for ribosome assembly.

Aberrant NOLC1 function links to cancer and neurodegenerative diseases. Elevated NOLC1 levels sometimes correlate with increased tumor growth connecting it to malignant processes. Its interactions with proteins such as tumor protein p53 highlight its importance in regulating cell cycle and stress responses. Additionally disruptions in its nucleolar roles have potential associations with neurodegenerative disorders indicating its critical role beyond normal cellular functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed : 10567578, PubMed : 26399832). Required for neural crest specification : following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed : 26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed : 9016786). It has intrinsic GTPase and ATPase activities (PubMed : 9016786).
See full target information NOLC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1312 PubMed38346978

2024

Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Licaj,Rana Mhaidly,Yann Kieffer,Hugo Croizer,Claire Bonneau,Arnaud Meng,Lounes Djerroudi,Kevin Mujangi-Ebeka,Hocine R Hocine,Brigitte Bourachot,Ilaria Magagna,Renaud Leclere,Lea Guyonnet,Mylene Bohec,Coralie Guérin,Sylvain Baulande,Maud Kamal,Christophe Le Tourneau,Fabrice Lecuru,Véronique Becette,Roman Rouzier,Anne Vincent-Salomon,Geraldine Gentric,Fatima Mechta-Grigoriou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com