AB241068

Anti-non-muscle Myosin IIA antibody

Name: Anti-non-muscle Myosin IIA antibody (ab241068) | Abcam
Brand: Abcam Limited
SKU: AB241068
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MYH9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Myosin-9 aa 1900-1950.

View Alternative Names

Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9

2 Images

Supplier Data

Immunoprecipitation - Anti-non-muscle Myosin IIA antibody (AB241068)

Non-muscle Myosin IIA was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

Lane 1 : ab241068 used for IP at 6 μg per reaction. For WB 1 μg/ml.
Lane 2 : Control IgG IP.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-non-muscle Myosin IIA antibody (ab241068)

Predicted band size: 227 kDa

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Supplier Data

Western blot - Anti-non-muscle Myosin IIA antibody (AB241068)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody (ab241068) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 227 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Myosin-9 aa 1900-1950. The exact immunogen used to generate this antibody is proprietary information.

P35579

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to non-muscle Myosin IIA immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.

Biological function summary

Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.

Pathways

Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.

Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed : 16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed : 20052411).. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.

See full target information Myosin-9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:1521-1538 PubMed41039173

2025

ANTXR1 blockade enhances cardiac function in preclinical models of heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Boccella,GuoJun Yu,Steven Seaman,Yang Feng,Jaewon Lee,Francesco Tomassoni-Ardori,Liping Yang,Kuo-Sheng Hsu,James M Dunleavey,Jodi Becker,Mary Beth Hilton,Karen Morris,Niza Borchin,Daeho So,Pradip Bajgain,Sudhirkumar Yanpallewar,Ryan T Gross,Krish C Dewan,Dawn E Bowles,Darren A Yuen,Lino Tessarollo,Brad St Croix

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-non-muscle Myosin IIA antibody

Immunoprecipitation - Anti-non-muscle Myosin IIA antibody (AB241068)

Western blot - Anti-non-muscle Myosin IIA antibody (AB241068)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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