Anti-non-muscle Myosin IIA antibody

• WB

Lab

Western blot - Anti-non-muscle Myosin IIA antibody (AB75590)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : non-muscle Myosin IIA knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK-293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab75590 observed at 230 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab75590 was shown to recognize non-muscle Myosin IIA in wild-type HAP1 cells along with additonal cross-reactive bands. No band was observed when non-muscle Myosin IIA knockout samples examined. Wild-type and non-muscle Myosin IIA knockout samples were subjected to SDS-PAGE. ab75590 at a concentration of 1 μg/ml and ab18058 (loading control to Vinculin) at a dilution of 1/1,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody (ab75590)

Predicted band size: 227 kDa

false

• WB

Unknown

Western blot - Anti-non-muscle Myosin IIA antibody (AB75590)

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody (ab75590) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate at 10 µg

Lane 4:

HEK-293 (Human embryonic kidney cell line) whole cell lysate at 10 µg

Lane 5:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 227 kDa

Observed band size: 100 kDa,227 kDa,35 kDa,45 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Anti-non-muscle Myosin IIA antibody (AB75590)

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody (ab75590) at 1 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse lung tissue lysate at 10 µg

Lane 4:

PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 227 kDa

Observed band size: 227 kDa,35 kDa,43 kDa

true

Exposure time: 1min