Rabbit Recombinant Monoclonal MYH9 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Images
![Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunoprecipitation-img3664.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)")
![Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--western-blot-img2373.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116703.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)")
Publications
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- PloS one 18:e02852512023HER2 inhibition increases non-muscle myosin IIA to promote tumorigenesis in HER2+ breast cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamar M Alanazi,Wasim Feroz,Rosalin Mishra,Mary Kate Kilroy,Hima Patel,Long Yuan,Sarah J Storr,Joan T GarrettPubMed 37200287
- Frontiers in immunology 14:10980562023Characteristic gene expression in the liver monocyte-macrophage-DC system is associated with the progression of fibrosis in NASH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxiao Wang,Zilong Wang,Baiyi Liu,Rui Jin,Yuyun Song,Ran Fei,Xu Cong,Rui Huang,Xiaohe Li,Jia Yang,Lai Wei,Huiying Rao,Feng LiuPubMed 36911682
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22035732023SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3β/β-Catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Li,Hao Luo,Fu-Qiang Dai,Ren-Tao Wang,Xiao-Qing Fan,Yuan-Yuan Luo,Meng-Sheng Deng,Yulun Wang,Tan Long,Wei Guo,Bo Xu,Cheng-Xiong Xu,Hua JinPubMed 36757050
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e22015412022Amphiphilic Aminated Derivatives of [60]Fullerene as Potent Inhibitors of Tumor Growth and Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawei Huo,Jie Li,Yang Liu,Libin Yang,Xinran Cao,Chong Zhao,Yicheng Lu,Wei Zhou,Shumu Li,Jianan Liu,Jiao Li,Xing Li,Jing Wan,Rui Wen,Mingming Zhen,Chunru Wang,Chunli BaiPubMed 36031401
- Journal of nanobiotechnology 19:1942021Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenglong Li,Jie Liu,Siyu Liu,Weijie Jiao,Xiaohong WangPubMed 34193158
- Frontiers in pharmacology 12:6982752021Matrix Stiffness Induces Pericyte-Fibroblast Transition Through YAP Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Feng,Xueyan Feng,Di Zhang,Qilong Li,Li YaoPubMed 34135765
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Not recommended | Expected |
| Mouse | Tested | Not recommended | Tested | Not recommended | Tested |
| Rat | Expected | Not recommended | Tested | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes IHC is not recommended for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes IHC is not recommended for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411). (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.
Alternative names
Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9
Recommended products
Rabbit Recombinant Monoclonal MYH9 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22933-9
- Purification technique
- Affinity purification Protein A
- Specificity
IHC is not recommended for human.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.
- Biological function summary
Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.
- Pathways
Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.
- Associated diseases and disorders
Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunoprecipitation-img3664.jpg "Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)")
Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238131. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: ab238131 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab238131 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
Predicted band size: 227 kDa
Observed band size: 226 kDa
![Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--western-blot-img2373.jpg "Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)")
Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131) at 1/1000 dilution
Lane 1: Human liver tissue lysate 20ug
Lane 2: Human lung lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 4: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 5: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6: Mouse lung tissue lysate at 20 µg
Lane 7: Rat spleen tissue lysate at 20 µg
Lane 8: Rat lung tissue lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 4 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 227 kDa
Observed band size: 226 kDa
Exposure time: 26s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-ab238131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116703.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
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