Anti-non-muscle Myosin IIA antibody [EPR22933-9]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver (PMID : 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver (PMID : 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IP

Unknown

Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)

non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238131. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug

Lane 2 : ab238131 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238131 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

Predicted band size: 227 kDa

Observed band size: 226 kDa

false

• WB

Lab

Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (AB238131)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131) at 1/1000 dilution

Lane 1:

Human liver tissue lysate 20ug

Lane 2:

Human lung lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 4:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

Mouse lung tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 4 - 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 227 kDa

Observed band size: 226 kDa

false

Exposure time: 26s