Rabbit Recombinant Monoclonal MYH9 antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunoprecipitation-img3664.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116703.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (AB256477)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | IP | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Predicted | Predicted |
| Mouse | Not recommended | Expected | Not recommended | Tested | Tested |
| Rat | Not recommended | Expected | Not recommended | Expected | Tested |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes IHC is not recommended for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes IHC is not recommended for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411). (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.
Alternative names
Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9
Recommended products
Rabbit Recombinant Monoclonal MYH9 antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22933-9
- Purification technique
- Affinity purification Protein A
- Specificity
IHC is not recommended for human.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256477 is the carrier-free version of Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.
- Biological function summary
Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.
- Pathways
Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.
- Associated diseases and disorders
Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunoprecipitation-img3664.jpg "Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)")
Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131).
All lanes: Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131)
Predicted band size: 227 kDa
Observed band size: 226 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat liver (PMID: 11943476, 22558294, 17668046). The section was incubated with Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477), expandable thumbnail](https://content.abcam.com/products/images/non-muscle-myosin-iia-antibody-epr22933-9-bsa-and-azide-free-ab256477--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116703.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse liver (PMID: 11943476, 22558294, 17668046). The section was incubated with Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-non-muscle Myosin IIA antibody [EPR22933-9] ab238131).
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