AB138498

Anti-non-muscle Myosin IIA antibody [EPR8965]

Name: Anti-non-muscle Myosin IIA antibody [EPR8965] (ab138498) | Abcam
Brand: Abcam Limited
SKU: AB138498
Price: 440 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(21 Publications)

Rabbit Recombinant Monoclonal MYH9 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 21 publications.

View Alternative Names

Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunofluorescent analysis of HeLa cells labelling non-muscle Myosin IIA with ab138498 at 1/250 dilution.

Unknown

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (ab138498) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

HUVEC lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

A431 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 227 kDa

Observed band size: 230 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling non-muscle Myosin IIA with ab138498 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling non-muscle Myosin IIA with purified ab138498 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunofluorescent analysis of A431 cells labelling non-muscle Myosin IIA with ab138498 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling non-muscle Myosin IIA with ab138498 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : non-muscle Myosin IIA knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab138498 observed at 230 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab138498 was shown to specifically react with non-muscle Myosin IIA in wild-type HAP1 cells. No band was observed when non-muscle Myosin IIA knockout samples were examined. Wild-type and non-muscle Myosin IIA knockout samples were subjected to SDS-PAGE. ab138498 at a dilution of 1/1000 and ab18058 (loading control to Vinculin) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (ab138498)

Predicted band size: 227 kDa

false

HRP

HRP Anti-non-muscle Myosin IIA antibody [EPR8965]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-non-muscle Myosin IIA antibody [EPR8965]
578 PE

PE Anti-non-muscle Myosin IIA antibody [EPR8965]
Carrier free

Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-non-muscle Myosin IIA antibody [EPR8965]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8965

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.

Biological function summary

Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.

Pathways

Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.

Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed : 16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed : 20052411).. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.

See full target information Myosin-9

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

International journal of ophthalmology 18:969-977 PubMed40534807

2025

Human congenital cataract mutation in alters F-actin organization and cell functions.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yuan,Zhen-Yu Wang,Jia-Rui Yang,Chen Huang,Liang Zhu,Xue-Min Li

Nature communications 16:3419 PubMed40210858

2025

A TLK2-mediated calcium-driven cell death pathway links neuronal degeneration to nuclear envelope disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Yajie Li,Huaiyuan Huang,Jingwen Gao,Jinhong Lu,Guifeng Kang,Yipeng Ge,Wencan Jiang,Xiang Cai,Guojun Zhang,Lei Liu

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette

Frontiers in veterinary science 11:1428273 PubMed39568484

2024

Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Chen,Na Chen,Hongbo Chen,Zefang Zhao,Jiayao Yang,Jianbo Sun,Hanmei Li,Rihua Cong,Hailong Liu,Tengfei Liu,Shulin Chen

British journal of cancer 131:1591-1604 PubMed39390252

2024

Quiescent cancer cells induced by high-density cultivation reveals cholesterol-mediated survival and lung metastatic traits.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyang Liu,Qinjie Min,Xinxin Cheng,Weimin Zhang,Qingnan Wu,Xu Chen,Mengzhu Lv,Siqi Liu,Huihui Zhao,Di Yang,Yidi Tai,Xiao Lei,Yan Wang,Qimin Zhan

Microsystems & nanoengineering 10:97 PubMed39015940

2024

Dynamic nanomechanical characterization of cells in exosome therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Chen,Zihan Zhang,Ziwei Li,Wenjie Wu,Shihai Lan,Tianhao Yan,Kainan Mei,Zihan Qiao,Chen Wang,Chuanbiao Bai,Ziyan Li,Shangquan Wu,Jianye Wang,Qingchuan Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401478 PubMed38785178

2024

Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Yazhou Chen,Qizheng Zhang,Shenyu Yang,Guanying Li,Chaochen Shi,Xunwu Hu,Shunsuke Asahina,Natsuko Asano,Ye Zhang

Discover oncology 15:88 PubMed38536591

2024

Circ_0020460 drives tumorigenesis in cervical cancer through miR-485-3p sponging.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yan,Chunyan Hu,Yali Cheng,Lingzhi Zheng,Baojin Zeng,Sujun Zhao,Chen Liu

Molecular medicine reports 29: PubMed38099337

2023

Exploring the regulatory role of Linc00893 in asthenozoospermia: Insights into sperm motility and SSC viability.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Lu,Dongchuan Xu,Liqiang Zhao,Hailing Ruan,Anguo Wang,Jiajia Hu,Meifang Xiao,Weiying Lu

International journal of molecular sciences 24: PubMed37108645

2023

Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dipali Goyal,Sean W Limesand,Ravi Goyal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-non-muscle Myosin IIA antibody [EPR8965]

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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