AB236073

Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal MYH9 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunofluorescent analysis of HeLa cells labelling non-muscle Myosin IIA with ab138498 at 1/250 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138498).

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

This data was developed using ab138498, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling non-muscle Myosin IIA with purified ab138498 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling non-muscle Myosin IIA with ab138498 antibody at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138498).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling non-muscle Myosin IIA with ab138498 antibody at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138498).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunofluorescent analysis of A431 cells labelling non-muscle Myosin IIA with ab138498 at 1/250 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138498).

Lab

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : non-muscle Myosin IIA knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab138498 observed at 230 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab138498 was shown to specifically react with non-muscle Myosin IIA in wild-type HAP1 cells. No band was observed when non-muscle Myosin IIA knockout samples were examined. Wild-type and non-muscle Myosin IIA knockout samples were subjected to SDS-PAGE. ab138498 at a dilution of 1/1000 and ab18058 (loading control to Vinculin) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138498).

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] (<a href='/en-us/products/primary-antibodies/non-muscle-myosin-iia-antibody-epr8965-ab138498'>ab138498</a>)

Predicted band size: 227 kDa

false

Unconjugated

Anti-non-muscle Myosin IIA antibody [EPR8965]
HRP

HRP Anti-non-muscle Myosin IIA antibody [EPR8965]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-non-muscle Myosin IIA antibody [EPR8965]
578 PE

PE Anti-non-muscle Myosin IIA antibody [EPR8965]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-non-muscle Myosin IIA antibody [EPR8965]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8965

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab236073 is the carrier-free version of ab138498.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Non-muscle Myosin IIA also known as Myosin Heavy Chain 9 or Myosin-9 is a motor protein that enables cellular mechanical tasks such as actin filament cross-linking and contraction. It has a molecular mass of approximately 226 kDa. Myosin IIA is expressed in a variety of cell types including epithelial cells fibroblasts and leukocytes where it contributes to various cellular activities. Myosin staining often reveals its localization at the leading edge of migrating cells revealing its involvement in cell movement.

Biological function summary

Non-muscle Myosin IIA plays an essential role in maintaining cell shape and promoting cell division. It combines with other myosin proteins into a complex often in conjunction with actin filaments. This myosin protein group further facilitates processes like cytokinesis and cellular migration. Myosin IIA's complex interactions allow cells to adapt to different mechanical stresses ensuring that cells can move and divide properly within the tissue architecture.

Pathways

Non-muscle Myosin IIA participates in both the RhoA/ROCK pathway and the actin cytoskeleton regulation pathway. Through these pathways Myosin IIA interacts with other key proteins such as Rho-associated protein kinase to mediate several cellular functions like cell shape changes and motility. These interactions allow Myosin IIA to efficiently transmit mechanical signals and respond to extracellular stimuli.

Research links non-muscle Myosin IIA with specific conditions like cancer invasion and cardiovascular disorders. Abnormal function or expression of Myosin IIA can contribute to altered cell motility resulting in cancer metastasis. Additionally mutations or disruptions in Myosin IIA can associate with conditions such as thrombocytopenia. Proteins like integrins interact with Myosin IIA in these disease states highlighting their critical roles in pathological settings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed : 16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed : 20052411).. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.

See full target information Myosin-9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:785941 PubMed35111156

2022

Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Pesce,Nicola Manfrini,Chiara Cordiglieri,Spartaco Santi,Alessandra Bandera,Andrea Gobbini,Paola Gruarin,Andrea Favalli,Mauro Bombaci,Alessandro Cuomo,Federica Collino,Giulia Cricrì,Riccardo Ungaro,Andrea Lombardi,Davide Mangioni,Antonio Muscatello,Stefano Aliberti,Francesco Blasi,Andrea Gori,Sergio Abrignani,Raffaele De Francesco,Stefano Biffo,Renata Grifantini

British journal of pharmacology 178:2246-2265 PubMed33085791

2021

Regulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signalling via interaction with Gli1.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Feixia Wang,Jianlin He,Naqi Lian,Zhenyi Wang,Jiangjuan Shao,Hai Ding,Shanzhong Tan,Anping Chen,Zili Zhang,Shijun Wang,Shizhong Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

Western blot - Anti-non-muscle Myosin IIA antibody [EPR8965] - BSA and Azide free (AB236073)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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