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AB232975

Anti-non-muscle Myosin IIB/MYH10 antibody

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(1 Publication)

Rabbit Polyclonal MYH10 antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYH10 aa 400-750.

View Alternative Names

Myosin-10, Myosin heavy chain 10, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, MYH10

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

Formalin-fixed, paraffin-embedded human kidney tissue stained for non-muscle Myosin IIB/MYH10 using ab232975 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

Formalin-fixed, paraffin-embedded human liver tissue stained for non-muscle Myosin IIB/MYH10 using ab232975 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for non-muscle Myosin IIB/MYH10 using ab232975 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • WB

Supplier Data

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

All lanes:

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (ab232975) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 229 kDa

false

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • WB

Supplier Data

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

All lanes:

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (ab232975) at 2 µg/mL

All lanes:

Pig cerebrum lysate

Predicted band size: 229 kDa

false

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)
  • WB

Characteriser

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (AB232975)

All lanes:

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody (ab232975) at 2 µg/mL

All lanes:

Recombinant human non-muscle Myosin IIB/MYH10 protein

Predicted band size: 229 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MYH10 aa 400-750. The exact immunogen used to generate this antibody is proprietary information.

P35580

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Alternative Products

Primary Antibodies

AB230823

Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (AB230823)

  • 5
  • 1
Primary Antibodies

AB254472

Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - BSA and Azide free (AB254472)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB138498

Anti-non-muscle Myosin IIA antibody [EPR8965]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

  • 5
  • 1
Primary Antibodies

AB37484

Anti-Myosin heavy chain antibody [A4.1025]

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB50967

Anti-heavy chain cardiac Myosin antibody [BA-G5]

Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (AB50967)

  • 4
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Non-muscle Myosin IIB also known as MYH10 is a motor protein important in cell division and movement. This protein weighs approximately 228 kDa. Myosin IIB shows expression in various tissues including the brain heart and fibroblasts. Unlike other myosins found in muscle cells Myosin IIB functions mainly in non-muscle cells where it plays a role in organizing intracellular structure and shape.
Biological function summary

The non-muscle Myosin IIB contributes to cell motility and cytokinesis important for normal cell proliferation and maintenance. It operates as part of the actomyosin complex that includes actin and other myosin types. This complex facilitates contractile forces within cells enabling movements such as cell migration and tissue development. Myosin IIB works closely with actin filaments converting chemical energy from ATP into mechanical work required for these processes.

Pathways

Cell migration and tissue morphogenesis involve Myosin IIB. Myosin IIB participates in the Rho-ROCK signaling pathway which regulates actomyosin contraction during cellular movement and morphogenesis. It interacts with other myosins like Myosin IIA illustrating its role in shared pathways. These pathways control the balance between cellular adhesion and detachment necessary for proper cellular function.

Non-muscle Myosin IIB associates with neurological disorders and cancer. Changes in Myosin IIB function can disrupt normal cell division leading to cancer progression. In neurological disorders Myosin IIB affects cell signaling and structural integrity of neurons. For instance disruptions in interactions with actin and other related proteins like Myosin IIA and Tropomyosins can lead to these adverse biological effects showing its significance in maintaining healthy cellular operations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.
See full target information MYH10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102380 PubMed39640012

2024

A lipid nanoparticle-based oligodendrocyte-specific mRNA therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Sawamura,Kiyoshi Tachikawa,Rie Hikawa,Hisako Akiyama,Seiji Kaji,Ken Yasuda,Angel I Leu,Hyojung Hong,Rajesh Mukthavaram,Pad Chivukula,Hodaka Yamakado,Yoshio Hirabayashi,Ryosuke Takahashi,Shu-Ichi Matsuzawa
View all publications
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Product promise

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