Name: Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23]
SKU: ab230823
Rating: 5 (1 reviews)

Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) is a rabbit monoclonal antibody that is used to detect non-muscle Myosin IIB/MYH10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (AB230823), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Tested	Tested	Tested
Rat	Expected	Tested	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/600	Notes -
Species Human	Dilution info 1/600	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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2 products for Alternative Product

Target data

See full target information MYH10

Function

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B.

Alternative names

Myosin-10, Myosin heavy chain 10, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, MYH10

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22564-23
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of non-muscle Myosin IIB/MYH10?

Anti-non-muscle Myosin IIB/MYH10 [EPR22564-23] (ab230823) specifically detects a band for non-muscle Myosin IIB/MYH10 (UniProt: P35580) at a molecular weight of 229kDa.

Recommended positive controls

WB: NIH/3T3, HEK-293T, HeLa and A549 whole cell lysates; human liver tissue lysate; rat liver, brain and lung tissue lysates; mouse brain tissue lysate.IP: HEK-293T whole cell lysate.IHC-P: Human cerebellum and kidney tissue; mouse cerebellum tissue; rat cerebellum and kidney tissue.ICC/IF: HeLa and NIH/3T3 cells.Flow Cyt (intra): HEK-293T and NIH/3T3 cells.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EPR22564-23] also available for your convenience:
ab230823, Carrier free - ab254472, ab300647, Carrier free - ab300648, Carrier free - ab300649

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Non-muscle Myosin IIB also known as MYH10 is a motor protein important in cell division and movement. This protein weighs approximately 228 kDa. Myosin IIB shows expression in various tissues including the brain heart and fibroblasts. Unlike other myosins found in muscle cells Myosin IIB functions mainly in non-muscle cells where it plays a role in organizing intracellular structure and shape.

Biological function summary

The non-muscle Myosin IIB contributes to cell motility and cytokinesis important for normal cell proliferation and maintenance. It operates as part of the actomyosin complex that includes actin and other myosin types. This complex facilitates contractile forces within cells enabling movements such as cell migration and tissue development. Myosin IIB works closely with actin filaments converting chemical energy from ATP into mechanical work required for these processes.

Pathways

Cell migration and tissue morphogenesis involve Myosin IIB. Myosin IIB participates in the Rho-ROCK signaling pathway which regulates actomyosin contraction during cellular movement and morphogenesis. It interacts with other myosins like Myosin IIA illustrating its role in shared pathways. These pathways control the balance between cellular adhesion and detachment necessary for proper cellular function.

Associated diseases and disorders

Non-muscle Myosin IIB associates with neurological disorders and cancer. Changes in Myosin IIB function can disrupt normal cell division leading to cancer progression. In neurological disorders Myosin IIB affects cell signaling and structural integrity of neurons. For instance disruptions in interactions with actin and other related proteins like Myosin IIA and Tropomyosins can lead to these adverse biological effects showing its significance in maintaining healthy cellular operations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong positive staining on Purkinje cells of human cerebellum (PMID: 29330091) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab230823 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunoprecipitation - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
non-muscle Myosin IIB/MYH10 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab230823 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230823 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T whole cell lysate 10 μg (Input).
Lane 2: ab230823 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab230823 in HEK-293T whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 secs.
All lanes: Immunoprecipitation - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Predicted band size: 229 kDa
Observed band size: 230 kDa
Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 3.25 secs. Lane 4: 15 secs. Lanes 5 & 6: 3 mins.
All lanes: Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) at 1/2000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4: Human liver tissue lysate at 20 µg
Lane 5: Rat lung tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 229 kDa
Observed band size: 230 kDa
Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/600 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^ï¿½488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat renal tubules (PMID: 29208685) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab230823 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong positive staining on Purkinje cells of rat cerebellum (PMID: 29330091) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab230823 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong positive staining on Purkinje cells of mouse cerebellum (PMID: 29330091) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab230823 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human renal tubules (PMID: 29208685) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab230823 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/600 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^ï¿½ 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1 & 2: 15 secs. Lane 3: 5.5 secs.
All lanes: Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 229 kDa
Observed band size: 230 kDa
Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling non-muscle Myosin IIB/MYH10 with ab230823 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.