Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab305263, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/500 dilution (2.304 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebellum. The section was incubated with ab300647 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab305263, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/500 dilution (2.304 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab300647 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. non-muscle Myosin IIB/MYH10 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate, 10µg with ab300647 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300647 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution. Lane 1 : HEK-293T whole cell lysate, 10µg Lane 2 : ab300647 IP in HEK-293T whole cell lysate Lane 3 : Rat monoclonal IgG2a (ab18450) instead of ab300647 in HEK-293T whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/non-muscle-myosin-iib-myh10-antibody-epr22564-23-rat-igg2a-chimeric-ab300647'>ab300647</a>) at 1/30 dilution

All lanes:

HEK-293T (human embryonic kidney epithelial cell)

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Observed band size: 250 kDa

false

Exposure time: 1s

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/50 dilution (23.04 µg/ml), followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/mL) (Green). Confocal image showing cytoskeletal staining in NIH/3T3 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/mL) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab300647 at a 1/50 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/500 dilution (2.304 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebellum. The section was incubated with ab300647 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/500 dilution (2.304 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebellum. The section was incubated with ab300647 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a - Chimeric - BSA and Azide Free (AB300648)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1-2 : 10 seconds Lane 3-6 : 37 seconds

All lanes:

Western blot - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a - Chimeric (<a href='/en-us/products/primary-antibodies/non-muscle-myosin-iib-myh10-antibody-epr22564-23-rat-igg2a-chimeric-ab300647'>ab300647</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Mouse lung tissue lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 250 kDa

false