AB133726

Anti-NOP10 antibody [EPR8856]

Name: Anti-NOP10 antibody [EPR8856] (ab133726) | Abcam
Brand: Abcam Limited
SKU: AB133726
Price: 585 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal NOP10 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

NOLA3, NOP10, H/ACA ribonucleoprotein complex subunit 3, Nucleolar protein 10, Nucleolar protein family A member 3, snoRNP protein NOP10

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (AB133726)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (AB133726)

Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma tissue staining NOP10 using ab133726 at a 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (AB133726)

Immunohistochemical analysis of paraffin-embedded Human breast tissue staining NOP10 using ab133726 at a 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-NOP10 antibody [EPR8856] (AB133726)

All lanes:

Western blot - Anti-NOP10 antibody [EPR8856] (ab133726) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 7 kDa

false

OI-RD Scanning - Anti-NOP10 antibody [EPR8856] (AB133726)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NOP10 antibody [EPR8856] (AB133726)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-NOP10 antibody [EPR8856] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8856

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOP10 also known as Nucleolar Protein 10 is a small protein component with a molecular weight of approximately 7 kDa. It is an essential part of the H/ACA ribonucleoprotein complexes responsible for guiding the isomerization of uridine to pseudouridine in ribosomal RNA (rRNA). NOP10 is expressed in the nucleolus where it interacts closely with other core proteins such as NHP2 NOP58 and the catalytic subunit dyskerin also known as DKC1.

Biological function summary

NOP10 plays a critical role in ribosome biogenesis by taking part in H/ACA snoRNP complexes that facilitate the processing and modification of rRNA which is necessary for proper ribosome function. The protein forms a complex with the other core proteins and small nucleolar RNAs (snoRNAs) essential for the chemical alteration and folding of rRNA. By supporting the formation and stability of these complexes NOP10 ensures the fidelity of ribosomal assembly and cellular protein synthesis.

Pathways

The protein contributes significantly to the ribosome biogenesis and RNA modification pathways. It directly influences the ribosome biogenesis process which determines the production of ribosomes essential for protein translation in cells. In this pathway NOP10 interacts with proteins such as GAR1 and dyskerin which assist in assembling the complete ribosomal subunits. These interactions help sustain the delicate balance of rRNA modifications required for functional ribosome activity during protein synthesis.

NOP10 is associated with conditions like dyskeratosis congenita and some forms of cancer. Dyskeratosis congenita a genetic disorder characterized by bone marrow failure and skin abnormalities is linked to mutations in components of the H/ACA snoRNP complex including NOP10. In cancer altered expression or functionality of NOP10 can influence the process of ribosome biogenesis leading to increased cellular proliferation. Dyskerin another key member of the complex is also implicated in these conditions highlighting the intertwined role of these proteins in maintaining cellular homeostasis and their potential disruption in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA (PubMed : 32554502). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

See full target information NOP10

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 33:1199-1211 PubMed30132373

2018

Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Quan Wang,Meng Di Yang,Xing Chen,Yang Wang,Liu Zeng Chen,Xiu Cheng,Xin Hua Liu

Nature 557:190-195 PubMed29695869

2018

Cryo-EM structure of substrate-bound human telomerase holoenzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Hoang Duong Nguyen,Jane Tam,Robert A Wu,Basil J Greber,Daniel Toso,Eva Nogales,Kathleen Collins

eLife 3: PubMed25407680

2014

The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.

Applications

IHC

Species

Human

Yick W Fong,Jaclyn J Ho,Carla Inouye,Robert Tjian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOP10 antibody [EPR8856]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (AB133726)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (AB133726)

Western blot - Anti-NOP10 antibody [EPR8856] (AB133726)

OI-RD Scanning - Anti-NOP10 antibody [EPR8856] (AB133726)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise