Rabbit Recombinant Monoclonal NOP2 antibody. Carrier free. Suitable for ICC/IF, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--western-blot-img174800.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165893.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166857.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171635.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img177181.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (AB282822)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Expected | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA. Required for efficient rRNA processing and 60S ribosomal subunit biogenesis. Regulates pre-rRNA processing through non-catalytic complex formation with box C/D snoRNAs and facilitates the recruitment of U3 and U8 snoRNAs to pre-90S ribosomal particles and their stable assembly into snoRNP complexes. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.
Alternative names
Nol1, Nop2, 28S rRNA (cytosine-C(5))-methyltransferase, Nucleolar protein 1, Nucleolar protein 2 homolog, Proliferating-cell nucleolar antigen p120, Proliferation-associated nucleolar protein p120
Recommended products
Rabbit Recombinant Monoclonal NOP2 antibody. Carrier free. Suitable for ICC/IF, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24888-92
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282822 is the carrier-free version of Anti-NOP2 antibody [EPR24888-92] ab271075.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOP2 also known as NOP2 nucleolar protein is a methyltransferase enzyme involved in ribosomal RNA (rRNA) processing. It weighs approximately 82 kDa. NOP2 is widely expressed in various cell types particularly in rapidly dividing cells where ribosome biogenesis is active. The protein resides predominantly in the nucleolus where it assists in rRNA modification and proper ribosome assembly.
- Biological function summary
NOP2 plays an important role in the maturation of 28S rRNA during the biogenesis of the 60S ribosomal subunit. It functions as part of the larger ribosome assembly complex. By modifying rRNA NOP2 ensures correct rRNA folding and stabilization essential for the production of functional ribosomes. This enzymatic activity aligns with its critical role in the proliferation of cells impacting processes such as protein synthesis and cell growth.
- Pathways
NOP2 is intimately involved in the ribosome biogenesis and assembly pathway. It interacts with other ribosomal proteins and ribosome assembly factors such as NOP58 during ribosome maturation. NOP2's role extends into the mitotic cell cycle pathway where it influences cellular proliferation. Understanding its connection with these pathways supports insights into cellular growth regulation and transformation.
- Associated diseases and disorders
NOP2 associates strongly with cancer pathogenesis. Overexpression of NOP2 frequently occurs in various cancers suggesting a potential role in oncogenesis. Dysregulated ribosome biogenesis facilitated by NOP2 can lead to tumor progression and metastasis. The protein's interactions with oncoproteins such as c-Myc further emphasize its importance in understanding cancer mechanisms and therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--western-blot-img174800.jpg "Western blot - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Western blot - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:25043274).
The multiple reactive bands would represent different NOP2 isoforms.
All lanes: Western blot - Anti-NOP2 antibody [EPR24888-92] (Anti-NOP2 antibody [EPR24888-92] ab271075) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 89 kDa
Observed band size: 110 kDa, 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165893.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/200 dilution (2.835 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nucleolar staining on human cerebrum (PMID: 25481415). The section was incubated with Anti-NOP2 antibody [EPR24888-92] ab271075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166857.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/200 dilution (2.835 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human lung adenocarcinoma (PMID: 3422591). The section was incubated with Anti-NOP2 antibody [EPR24888-92] ab271075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171635.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/500 dilution (1.134 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nucleolar staining on mouse cerebrum. The section was incubated with Anti-NOP2 antibody [EPR24888-92] ab271075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img177181.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/500 dilution (1.134 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nucleolar staining on rat cerebrum. The section was incubated with Anti-NOP2 antibody [EPR24888-92] ab271075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunocytochemistry-immunofluorescence-img170353.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma cell line) cells labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/500 dilution (1.134 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml / Green). Confocal image showing nucleolar staining in HCT 116 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml / Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml) dilution.
![Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunoprecipitation-img173831.jpg "Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
NOP2 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2: Anti-NOP2 antibody [EPR24888-92] ab271075 IP in 293T whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NOP2 antibody [EPR24888-92] ab271075 in 293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
All lanes: Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] (Anti-NOP2 antibody [EPR24888-92] ab271075)
Predicted band size: 89 kDa
Observed band size: 110 kDa, 90 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunocytochemistry-immunofluorescence-img170838.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/500 dilution (1.134 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml / Green). Confocal image showing nucleolar staining in Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml / Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).
![Flow Cytometry (Intracellular) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--flow-cytometry-intracellular-img172063.jpg "Flow Cytometry (Intracellular) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
Flow Cytometry (Intracellular) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)
This data was developed using Anti-NOP2 antibody [EPR24888-92] ab271075, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling NOP2 with Anti-NOP2 antibody [EPR24888-92] ab271075 at 1/50 dilution (1μg / Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 / Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody / Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunocytochemistry-immunofluorescence-img170353.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
![Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunoprecipitation-img173831.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
![Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--immunocytochemistry-immunofluorescence-img170838.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
![Flow Cytometry (Intracellular) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822), expandable thumbnail](https://content.abcam.com/products/images/nop2-antibody-epr24888-92-bsa-and-azide-free-ab282822--flow-cytometry-intracellular-img172063.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NOP2 antibody [EPR24888-92] - BSA and Azide free (ab282822)")
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