AB236724

Anti-NOP58 antibody

Name: Anti-NOP58 antibody (ab236724) | Abcam
Brand: Abcam Limited
SKU: AB236724

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(2 Publications)

Rabbit Polyclonal NOP58 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NOP58.

View Alternative Names

NOL5, NOP5, HSPC120, NOP58, Nucleolar protein 58, Nucleolar protein 5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody (AB236724)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody (AB236724)

Paraffin-embedded human tonsil tissue stained for NOP58 using ab236724 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody (AB236724)

Paraffin-embedded human liver cancer tissue stained for NOP58 using ab236724 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody (AB236724)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody (AB236724)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOP58 (green) using ab236724 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-NOP58 antibody (AB236724)

All lanes:

Western blot - Anti-NOP58 antibody (ab236724) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NOP58.

Q9Y2X3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOP58 also known as Nop58p is a nucleolar protein involved in the biogenesis of small nucleolar ribonucleoproteins (snoRNPs). This protein weighs approximately 58 kDa. Researchers find NOP58 expressed in the nucleolus of eukaryotic cells. NOP58 is part of the box C/D small nucleolar RNA (snoRNA) complex which plays a critical role in the maturation and modification of pre-rRNA.

Biological function summary

NOP58 contributes to the assembly and function of snoRNPs which guide the chemical modification of rRNA particularly 2'-O-methylation. This modification has significance for proper ribosome assembly and function. NOP58 does not work alone; it forms part of a complex with other proteins such as NOP56 Fibrillarin and SNU13. These proteins come together to accurately target and chemically modify specific rRNA sequences.

Pathways

NOP58 participates in the ribosome biogenesis and rRNA processing pathways. These pathways are essential for producing the ribosomal units required for protein synthesis. NOP58 functions alongside proteins like NOP56 and Fibrillarin in modifying rRNA which is critical within the nucleolus. Proper functioning of these pathways ensures the cell maintains protein synthesis efficiency and accuracy.

Alterations in NOP58 may affect nucleolar function potentially leading to ribosomopathies. Disruptions in NOP58 can potentially associate with conditions such as cancer where nucleolar activity is often altered. Proteins like Fibrillarin associated with snoRNP complexes may also be implicated in these diseases as they share involvement in similar pathways and complex functions with NOP58.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs (PubMed : 15574333, PubMed : 17636026, PubMed : 19620283, PubMed : 34516797). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs) (PubMed : 39570315).

See full target information NOP58

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 25:35-48 PubMed38058606

2023

Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Zhenxiao Ren,Tianzuo Niu,Siyuan Li

Scientific reports 13:20730 PubMed38007504

2023

A1BG-AS1 promotes adriamycin resistance of breast cancer by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Jie Xu,Jie Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NOP58 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody (AB236724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody (AB236724)

Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody (AB236724)

Western blot - Anti-NOP58 antibody (AB236724)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise