Rabbit Recombinant Monoclonal Noradrenaline antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Chemical samples.
Images
![Western blot - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--western-blot-img426209.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970)")
![Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--immunocytochemistry-immunofluorescence-img426221.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970)")
![Indirect ELISA - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--indirect-elisa-img426249.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (AB315970)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | I-ELISA | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Chemical | Tested | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Chemical | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chemical, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Alternative names
Arterenol, NA
Recommended products
Rabbit Recombinant Monoclonal Noradrenaline antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Chemical samples.
Alternative names
Arterenol, NA
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24435-114
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab315970 is the carrier-free version of Anti-Noradrenaline antibody [EPR24435-114] ab315969.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Noradrenaline also known as norepinephrine is a neurotransmitter and hormone with a molecular weight of approximately 169.18 g/mol. It is primarily synthesized and released by neurons in the central nervous system and sympathetic nervous system from the adrenal medulla. Noradrenaline functions mechanically by acting on adrenergic receptors including alpha and beta subtypes which are distributed widely in the body. These receptors facilitate its major roles in various physiological responses such as modulation of heart rate focus and alertness.
- Biological function summary
Noradrenaline plays significant roles in the fight-or-flight response and regulation of mood and blood pressure. As part of the catecholamine family noradrenaline often works in conjunction with other neurotransmitters like dopamine. It is involved in the formation of synaptic networks and influences neuronal signaling through its action on adrenergic receptors located in multiple tissues including the heart vasculature and brain. It does not form part of a molecular complex but interacts dynamically with several proteins that mediate its effects.
- Pathways
Noradrenaline participates in the adrenergic signaling pathway influencing cardiovascular and metabolic processes. Within this pathway it regulates functions by interacting with proteins such as adrenergic receptors and modulates the release of neurotransmitters like serotonin. Additionally the stress response pathway also known as the hypothalamic-pituitary-adrenal axis significantly involves noradrenaline to regulate stress-related hormone synthesis and secretion affecting various bodily systems.
- Associated diseases and disorders
Noradrenaline shows connections to cardiovascular disorders and mood disorders such as depression. Abnormal noradrenaline levels can lead to hypertension by increasing peripheral vascular resistance alongside the involvement of proteins like beta-adrenergic receptors. In mood disorders dysregulation of noradrenaline along with dopamine and serotonin contributes to the pathophysiology of depression highlighting the complex neurochemical interactions affecting mental health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--western-blot-img426209.jpg "Western blot - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)")
Western blot - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)
This data was developed using Anti-Noradrenaline antibody [EPR24435-114] ab315969, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with Dopamine and Adrenaline.
In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] - (Anti-Albumin antibody [EPR12774] ab192603) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Noradrenaline antibody [EPR24435-114] (Anti-Noradrenaline antibody [EPR24435-114] ab315969) at 1/1000 dilution
Lane 1: Norepinephrine cross-linked to BSA at 20 ng
Lane 2: Dopamine cross-linked to BSA at 500 ng
Lane 3: Adrenaline cross-linked to BSA at 100 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 67 kDa
Exposure time: 10s
This antibody does not cross-react with Dopamine and Adrenaline.
In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] - (Anti-Albumin antibody [EPR12774] ab192603) staining at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--immunocytochemistry-immunofluorescence-img426221.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)")
Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)
This data was developed using Anti-Noradrenaline antibody [EPR24435-114] ab315969, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Noradrenaline with Anti-Noradrenaline antibody [EPR24435-114] ab315969 at 1/50 (10.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) antibody at 1/1000 (2ug/ml) dilution (Magenta).
Confocal image showing positive staining in RAW 264.7 cells treated with 0.5 mg/mL Norepinephrine-crosslink-BSA-FITC for 4 hours and no staining when 0.5 mg/mL crosslink-BSA-FITC was incubated for 4 hours.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The Nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at 1/1000 (2ug/ml) dilution.
![Indirect ELISA - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970), expandable thumbnail](https://content.abcam.com/products/images/noradrenaline-antibody-epr24435-114-bsa-and-azide-free-ab315970--indirect-elisa-img426249.jpg "Indirect ELISA - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)")
Indirect ELISA - Anti-Noradrenaline antibody [EPR24435-114] - BSA and Azide free (ab315970)
This data was developed using Anti-Noradrenaline antibody [EPR24435-114] ab315969, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Noradrenaline antibody [EPR24435-114] ab315969 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Norepinephrine cross-linked to BSA, Dopamine cross-linked to BSA, Adrenaline cross-linked to BSA.
Antigen concentration: 1000 ng/ml
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