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AB41559

Anti-Noradrenaline transporter antibody

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(1 Review)

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(6 Publications)

Rabbit Polyclonal Noradrenaline transporter antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SLC6A2.

View Alternative Names

NAT1, NET1, SLC6A5, SLC6A2, Sodium-dependent noradrenaline transporter, Norepinephrine transporter, Solute carrier family 6 member 2, NET

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline transporter antibody (AB41559)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Noradrenaline transporter antibody (AB41559)

ICC/IF image of ab41559 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41559, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-Noradrenaline transporter antibody (AB41559)
  • WB

Unknown

Western blot - Anti-Noradrenaline transporter antibody (AB41559)

Western Blot with rat brain cortical membranes

All lanes:

Western blot - Anti-Noradrenaline transporter antibody (ab41559) at 1/250 dilution

All lanes:

rat brain cortical membrane cell lysate at 70 µg

Predicted band size: 69 kDa

Observed band size: 28 kDa,32 kDa,80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SLC6A2. The exact immunogen used to generate this antibody is proprietary information.

P23975

Specificity

Ab41559 does not cross react with dopamine and serotonin transporters.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.8 Preservative: 0.02% Sodium azide Constituents: Tris glycine, 30% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The noradrenaline transporter also known as norepinephrine transporter (NET) is a protein that transports the neurotransmitter noradrenaline from the synaptic cleft back into the presynaptic neuron. This process is important for terminating noradrenaline signaling and maintaining neurotransmitter balance. The mass of the noradrenaline transporter is approximately 70 kDa. This transporter is mainly expressed in neuronal cells in the central and peripheral nervous systems as well as in the adrenal glands where noradrenaline plays an important role in response mechanisms.
Biological function summary

The noradrenaline transporter functions to regulate extracellular levels of noradrenaline which is essential for proper functioning of the sympathetic nervous system. It is vital in the reuptake mechanism that controls the duration and intensity of noradrenaline's action. The transporter forms part of a larger complex that involves various proteins and binding molecules that facilitate noradrenaline reuptake efficiently by ensuring synaptic concentration of needed ions.

Pathways

The activity of the noradrenaline transporter is an integral part of the adrenergic signaling pathway which is instrumental in the body's response to stress. This pathway includes complex interactions involving G protein-coupled receptors and proteins like dopamine transporter which sometimes parallel its action. The transporter is also involved in the catecholamine biosynthesis pathway which is responsible for the production and breakdown of neurotransmitters including adrenaline and dopamine.

Alterations in the expression or function of the noradrenaline transporter have been associated with conditions such as depression and attention-deficit hyperactivity disorder (ADHD). In these conditions altered transporter function can lead to neurotransmitter imbalance affecting mood and attention. Research has also indicated potential links with other proteins like serotonin transporter in mood disorders highlighting its involvement in broader neurochemical networks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (PubMed : 2008212, PubMed : 8125921). Can also mediate sodium- and chloride-dependent transport of dopamine (PubMed : 11093780, PubMed : 8125921).
See full target information SLC6A2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:6948 PubMed38521816

2024

Developmental expression of catecholamine system in the human placenta and rat fetoplacental unit.

Applications

Unspecified application

Species

Unspecified reactive species

Rona Karahoda,Veronika Vachalova,Ramon Portillo,Filip Mahrla,Mireia Viñas-Noguera,Cilia Abad,Frantisek Staud

Reproductive biology and endocrinology : RB&E 20:112 PubMed35927731

2022

Functional reorganization of monoamine transport systems during villous trophoblast differentiation: evidence of distinct differences between primary human trophoblasts and BeWo cells.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Vachalova,Rona Karahoda,Martina Ottaviani,Kasin Yadunandam Anandam,Cilia Abad,Christiane Albrecht,Frantisek Staud

Oncology letters 19:824-832 PubMed31897198

2020

Norepinephrine transporter promotes the invasion of human colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huahua Zhang,Jiyu Miao,Fang Li,Wanjuan Xue,Kaijie Tang,Xiaoge Zhao,Xintao Jing,Jing Zhang,Chen Huang,Ni Hou,Jiming Han

Physiological research 69:135-143 PubMed31852208

2019

Upregulation of gastric norepinephrine with beta-adrenoceptors and gastric dysmotility in a rat model of functional dyspepsia.

Applications

Unspecified application

Species

Unspecified reactive species

J Song,T Wang,X Zhang,B Li,C Zhu,S Zhang

Peptides 111:132-141 PubMed29969648

2018

Oxytocin modulates the expression of norepinephrine transporter, β-adrenoceptors and muscarinic M receptors in the hearts of socially isolated rats.

Applications

Unspecified application

Species

Unspecified reactive species

Predrag Jovanovic,Natasa Spasojevic,Nela Puskas,Bojana Stefanovic,Sladjana Dronjak

Experimental physiology 102:974-984 PubMed28590038

2017

Effects of oestrogen and Cimicifuga racemosa on the cardiac noradrenaline pathway of ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Liu,Jing Zhang,Shuya Liu,Wenjuan Wang,Xing Chen,Hai Jiang,Junlei Li,Ke Wang,Wenpei Bai,Haicheng Zhang,Lihua Qin
View all publications
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