AB211463

Anti-Noradrenaline transporter antibody [CL3063]

Name: Anti-Noradrenaline transporter antibody [CL3063] (ab211463) | Abcam
Brand: Abcam Limited
SKU: AB211463
Price: 395 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal Noradrenaline transporter antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC6A2 aa 150 to C-terminus.

View Alternative Names

NAT1, NET1, SLC6A5, SLC6A2, Sodium-dependent noradrenaline transporter, Norepinephrine transporter, Solute carrier family 6 member 2, NET

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placente tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human locus coeruleus tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat locus coeruleus tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse hippocampus tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling Noradrenaline transporter with ab211463 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3063

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC6A2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23975

Epitope

This antibody binds to an epitope located within the peptide sequence HTKYSKYKFTPAAEF (aa 199-213)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The noradrenaline transporter also known as norepinephrine transporter (NET) is a protein that transports the neurotransmitter noradrenaline from the synaptic cleft back into the presynaptic neuron. This process is important for terminating noradrenaline signaling and maintaining neurotransmitter balance. The mass of the noradrenaline transporter is approximately 70 kDa. This transporter is mainly expressed in neuronal cells in the central and peripheral nervous systems as well as in the adrenal glands where noradrenaline plays an important role in response mechanisms.

Biological function summary

The noradrenaline transporter functions to regulate extracellular levels of noradrenaline which is essential for proper functioning of the sympathetic nervous system. It is vital in the reuptake mechanism that controls the duration and intensity of noradrenaline's action. The transporter forms part of a larger complex that involves various proteins and binding molecules that facilitate noradrenaline reuptake efficiently by ensuring synaptic concentration of needed ions.

Pathways

The activity of the noradrenaline transporter is an integral part of the adrenergic signaling pathway which is instrumental in the body's response to stress. This pathway includes complex interactions involving G protein-coupled receptors and proteins like dopamine transporter which sometimes parallel its action. The transporter is also involved in the catecholamine biosynthesis pathway which is responsible for the production and breakdown of neurotransmitters including adrenaline and dopamine.

Alterations in the expression or function of the noradrenaline transporter have been associated with conditions such as depression and attention-deficit hyperactivity disorder (ADHD). In these conditions altered transporter function can lead to neurotransmitter imbalance affecting mood and attention. Research has also indicated potential links with other proteins like serotonin transporter in mood disorders highlighting its involvement in broader neurochemical networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline), the primary signaling neurotransmitter in the autonomic sympathetic nervous system (PubMed : 2008212, PubMed : 8125921, PubMed : 38750358). Is responsible for norepinephrine re-uptake and clearance from the synaptic cleft, thus playing a crucial role in norepinephrine inactivation and homeostasis (By similarity). Can also mediate sodium- and chloride-dependent transport of dopamine (PubMed : 11093780, PubMed : 8125921, PubMed : 39395208, PubMed : 39048818).

See full target information SLC6A2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6264 PubMed39048565

2024

Pannexin-1 channel inhibition alleviates opioid withdrawal in rodents by modulating locus coeruleus to spinal cord circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Charlie H T Kwok,Erika K Harding,Nicole E Burma,Tamara Markovic,Nicolas Massaly,Nynke J van den Hoogen,Sierra Stokes-Heck,Eder Gambeta,Kristina Komarek,Hye Jean Yoon,Kathleen E Navis,Brendan B McAllister,Julia Canet-Pons,Churmy Fan,Rebecca Dalgarno,Evgueni Gorobets,James W Papatzimas,Zizhen Zhang,Yuta Kohro,Connor L Anderson,Roger J Thompson,Darren J Derksen,Jose A Morón,Gerald W Zamponi,Tuan Trang

Nature biotechnology : PubMed37974010

2023

Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Tao,Xueyan Li,Qiping Dong,Linghai Kong,Andrew J Petersen,Yuanwei Yan,Ke Xu,Seth Zima,Yanru Li,Danielle K Schmidt,Melvin Ayala,Sakthikumar Mathivanan,Andre M M Sousa,Qiang Chang,Su-Chun Zhang

Neurobiology of disease 173:105831 PubMed35908744

2022

Loss of mitochondrial enzyme GPT2 causes early neurodegeneration in locus coeruleus.

Applications

Unspecified application

Species

Unspecified reactive species

Ozan Baytas,Julie A Kauer,Eric M Morrow

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

International journal of molecular sciences 22: PubMed34206048

2021

Nrf2 Activation Attenuates Acrylamide-Induced Neuropathy in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chand Basha Davuljigari,Frederick Adams Ekuban,Cai Zong,Alzahraa A M Fergany,Kota Morikawa,Gaku Ichihara

Journal of neuroinflammation 18:10 PubMed33407625

2021

Comparisons of neuroinflammation, microglial activation, and degeneration of the locus coeruleus-norepinephrine system in APP/PS1 and aging mice.

Applications

Unspecified application

Species

Unspecified reactive species

Song Cao,Daniel W Fisher,Guadalupe Rodriguez,Tian Yu,Hongxin Dong

Molecular medicine reports 23: PubMed33215216

2020

Osteoglycin knockdown promotes vascular smooth muscle cell proliferation and migration in aortic dissection via the VEGF/VEGFR2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zanxin Wang,Xianmian Zhuang,Bailang Chen,Minxin Wei

Frontiers in neuroscience 14:151 PubMed32180699

2020

Role of Neuro-Immune Cross-Talk in the Anti-obesity Effect of Electro-Acupuncture.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjiang Lu,Yan He,Meirong Gong,Qian Li,Qianqian Tang,Xuan Wang,Yaling Wang,Mengqian Yuan,Zhi Yu,Bin Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Noradrenaline transporter antibody [CL3063]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [CL3063] (AB211463)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise