AB254361

Anti-Noradrenaline transporter antibody [EPR23527-534]

BOND RX™ Validated
20ul selling size
Recombinant
Advanced Validation
RabMAb
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) is a rabbit monoclonal antibody detecting Noradrenaline transporter in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

NAT1, NET1, SLC6A5, SLC6A2, Sodium-dependent noradrenaline transporter, Norepinephrine transporter, Solute carrier family 6 member 2, NET

22 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Positive staining in human cerebrum.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/500 dilution (C), and ARMET/ARP with ab316935 at a 1/1000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on human cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in human cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in human cerebrum.
Panel D : anti-ARMET/ARP staining neurons in human cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative control : no staining in rat hepatocytes.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative control : no staining in mouse hepatocytes.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Flow cytometric analysis of rat primary neuron cells labelling Noradrenaline transporter with ab254361 at 1/50 dilution (1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor^® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Positive staining in rat cerebrum.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

Positive staining on rat cerebrum is observed.

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

Positive staining on mouse cerebrum is observed.

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

Negative control : No staining on rat liver (PMID : 12807698) is observed.

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebellum.

Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D : anti-FMRP staining neurons in mouse cerebellum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebrum.

Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D : anti-FMRP staining neurons in mouse cerebrum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebellum.

Panel B : anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D : anti-FMRP staining neurons in rat cerebellum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.
Panel D : anti-FMRP staining neurons in rat cerebrum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Positive staining in mouse cerebrum.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

Negative control : No staining on mouse liver (PMID : 12807698) is observed.

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Lab

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : 293T. In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution. Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, anti-GAPDH antibody (ab181602) staining at 1/200,000 dilution and anti-Integrin alpha V (ab179475) staining at 1/5,000 dilution.

Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution

Lane 1:

Mouse brain membrane tissue lysate at 20 µg

Lane 2:

Mouse membrane-depleted tissue lysate at 20 µg

Lane 3:

Rat brain membrane tissue lysate at 20 µg

Lane 4:

Rat membrane-depleted tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : mouse skeletal muscle and rat liver. In Western blot, anti-GAPDH antibody (ab181602) was used as a GAPDH loading control and staining at 1/200, 000 dilution. Samples are non-boiled as boiling may cause protein aggregation

All lanes:

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat dorsal ganglion tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 180s

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
603 Alexa Fluor® 568

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23527-534

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, IHC-P, WB, mIHC, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/100", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/100", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" }, "Rat": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/100", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

What is the molecular weight of Noradrenaline transporter?
Anti-Noradrenaline transporter [EPR23527-534] (ab254361) specifically detects a band for Noradrenaline transporter (UniProt: O55192) at a molecular weight of 69kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23527-534] also available for your convenience: ab254361, Carrier free - ab282022

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The noradrenaline transporter also known as norepinephrine transporter (NET) is a protein that transports the neurotransmitter noradrenaline from the synaptic cleft back into the presynaptic neuron. This process is important for terminating noradrenaline signaling and maintaining neurotransmitter balance. The mass of the noradrenaline transporter is approximately 70 kDa. This transporter is mainly expressed in neuronal cells in the central and peripheral nervous systems as well as in the adrenal glands where noradrenaline plays an important role in response mechanisms.

Biological function summary

The noradrenaline transporter functions to regulate extracellular levels of noradrenaline which is essential for proper functioning of the sympathetic nervous system. It is vital in the reuptake mechanism that controls the duration and intensity of noradrenaline's action. The transporter forms part of a larger complex that involves various proteins and binding molecules that facilitate noradrenaline reuptake efficiently by ensuring synaptic concentration of needed ions.

Pathways

The activity of the noradrenaline transporter is an integral part of the adrenergic signaling pathway which is instrumental in the body's response to stress. This pathway includes complex interactions involving G protein-coupled receptors and proteins like dopamine transporter which sometimes parallel its action. The transporter is also involved in the catecholamine biosynthesis pathway which is responsible for the production and breakdown of neurotransmitters including adrenaline and dopamine.

Alterations in the expression or function of the noradrenaline transporter have been associated with conditions such as depression and attention-deficit hyperactivity disorder (ADHD). In these conditions altered transporter function can lead to neurotransmitter imbalance affecting mood and attention. Research has also indicated potential links with other proteins like serotonin transporter in mood disorders highlighting its involvement in broader neurochemical networks.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline), the primary signaling neurotransmitter in the autonomic sympathetic nervous system (PubMed : 2008212, PubMed : 8125921, PubMed : 38750358). Is responsible for norepinephrine re-uptake and clearance from the synaptic cleft, thus playing a crucial role in norepinephrine inactivation and homeostasis (By similarity). Can also mediate sodium- and chloride-dependent transport of dopamine (PubMed : 11093780, PubMed : 8125921, PubMed : 39395208, PubMed : 39048818).

See full target information SLC6A2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:648 PubMed41074162

2025

Fe-MOF loaded with capmatinib to reverse immunosuppressive microenvironment and inhibit tumor metastasis after radiofrequency ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Zhao,Lei Lei,Cheng Zeng,Xianfei Zhou,Junkang Ding,Wenbin Dai,Angfeng Jiang,Shaomin Zou,Qiao Jin,Zhe Tang

Nature communications 16:7338 PubMed40781079

2025

Early Locus Coeruleus noradrenergic axon loss drives olfactory dysfunction in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Meyer,Theresa Niedermeier,Paul L C Feyen,Felix L Strübing,Boris-Stephan Rauchmann,Katerina Karali,Johanna Gentz,Yannik E Tillmann,Nicolas F Landgraf,Svenja-Lotta Rumpf,Katharina Ochs,Karin Wind-Mark,Gloria Biechele,Jessica Wagner,Selim Guersel,Carolin I Kurz,Meike Schweiger,Danilo Prtvar,Yuan Shi,Richard B Banati,Guo-Jun Liu,Ryan J Middleton,Gerda Mitteregger-Kretzschmar,Robert Perneczky,Thomas Koeglsperger,Jonas J Neher,Sabina Tahirovic,Matthias Brendel,Jochen Herms,Lars Paeger

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Noradrenaline transporter antibody [EPR23527-534]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise