Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Rabbit Recombinant Monoclonal Noradrenaline transporter antibody. Suitable for mIHC, Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.

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Images

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (AB254361), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IP	Flow Cyt	WB	IHC-P	IHC-Fr
Human	Tested	Not recommended	Not recommended	Tested	Tested	Expected
Mouse	Tested	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Tested	Not recommended	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information SLC6A2

Function

Mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (PubMed:2008212, PubMed:8125921). Can also mediate sodium- and chloride-dependent transport of dopamine (PubMed:11093780, PubMed:8125921).

Alternative names

NAT1, NET1, SLC6A5, SLC6A2, Sodium-dependent noradrenaline transporter, Norepinephrine transporter, Solute carrier family 6 member 2, NET

Recommended products

Rabbit Recombinant Monoclonal Noradrenaline transporter antibody. Suitable for mIHC, Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23527-534
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The noradrenaline transporter also known as norepinephrine transporter (NET) is a protein that transports the neurotransmitter noradrenaline from the synaptic cleft back into the presynaptic neuron. This process is important for terminating noradrenaline signaling and maintaining neurotransmitter balance. The mass of the noradrenaline transporter is approximately 70 kDa. This transporter is mainly expressed in neuronal cells in the central and peripheral nervous systems as well as in the adrenal glands where noradrenaline plays an important role in response mechanisms.

Biological function summary

The noradrenaline transporter functions to regulate extracellular levels of noradrenaline which is essential for proper functioning of the sympathetic nervous system. It is vital in the reuptake mechanism that controls the duration and intensity of noradrenaline's action. The transporter forms part of a larger complex that involves various proteins and binding molecules that facilitate noradrenaline reuptake efficiently by ensuring synaptic concentration of needed ions.

Pathways

The activity of the noradrenaline transporter is an integral part of the adrenergic signaling pathway which is instrumental in the body's response to stress. This pathway includes complex interactions involving G protein-coupled receptors and proteins like dopamine transporter which sometimes parallel its action. The transporter is also involved in the catecholamine biosynthesis pathway which is responsible for the production and breakdown of neurotransmitters including adrenaline and dopamine.

Associated diseases and disorders

Alterations in the expression or function of the noradrenaline transporter have been associated with conditions such as depression and attention-deficit hyperactivity disorder (ADHD). In these conditions altered transporter function can lead to neurotransmitter imbalance affecting mood and attention. Research has also indicated potential links with other proteins like serotonin transporter in mood disorders highlighting its involvement in broader neurochemical networks.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

22 product images

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Flow cytometric analysis of rat primary neuron cells labelling Noradrenaline transporter with ab254361 at 1/50 dilution (1μg)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor^® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Positive staining in mouse cerebrum.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Positive staining on rat cerebrum is observed.
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Positive staining in rat cerebrum.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Positive staining on mouse cerebrum is observed.
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Positive staining in human cerebrum.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining in rat hepatocytes.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on rat liver (PMID: 12807698) is observed.
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on mouse liver (PMID: 12807698) is observed.
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling Noradrenaline transporter with ab254361 at 1/100 (5.33 μg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining in mouse hepatocytes.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: mouse skeletal muscle and rat liver.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) was used as a GAPDH loading control and staining at 1/200, 000 dilution.
Samples are non-boiled as boiling may cause protein aggregation
All lanes: Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution
Lane 1: Mouse cerebral cortex tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat dorsal ganglion tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 180s
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.

Panel D: anti-ARMET/ARP staining neurons in rat cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200, 000 dilution and anti-Integrin alpha V (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5, 000 dilution.
Samples are non-boiled as boiling may cause protein aggregation.
All lanes: Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution
Lane 1: Mouse brain membrane tissue lysate at 20 µg
Lane 2: Mouse brain membrane control tissue lysate at 20 µg
Lane 3: Rat brain membrane tissue lysate at 20 µg
Lane 4: Rat brain membrane control tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 180s
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at a 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/1000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on human cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in human cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in human cerebrum.

Panel D: anti-ARMET/ARP staining neurons in human cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D: anti-ARMET/ARP staining neurons in mouse cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D: anti-ARMET/ARP staining neurons in rat cerebellum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D: anti-ARMET/ARP staining neurons in mouse cerebellum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D: anti-FMRP staining neurons in mouse cerebellum.

The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D: anti-FMRP staining neurons in rat cerebellum.

The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.

Panel D: anti-FMRP staining neurons in rat cerebrum.

The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D: anti-FMRP staining neurons in mouse cerebrum.

The section was incubated in three rounds of staining: in the order of ab254361, Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: 293T.
In Western blot, anti-Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10, 000 dilution.
Samples are non-boiled as boiling may cause protein aggregation.
All lanes: Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 180s

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This is Abcam

Anti-Noradrenaline transporter antibody [EPR23527-534]

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Immunogen

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mIHC

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Flow Cyt

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WB

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IHC-P

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IHC-Fr

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22 product images

Flow Cytometry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Frozen sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Multiplex immunohistochemistry - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (ab254361)

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Not recommended

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Not recommended
Not recommended

Tested
Tested

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Expected