AB224451

Anti-Norbin antibody

Name: Anti-Norbin antibody (ab224451) | Abcam
Brand: Abcam Limited
SKU: AB224451

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(1 Publication)

Rabbit Polyclonal Norbin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCDN aa 600 to C-terminus.

View Alternative Names

KIAA0607, NCDN, Neurochondrin

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Norbin antibody (AB224451)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Norbin antibody (AB224451)

PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Norbin (green) using ab224451 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norbin antibody (AB224451)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norbin antibody (AB224451)

Paraffin embedded human cerebral cortex tissue stained for Norbin with ab224451 (1/500 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Norbin antibody (AB224451)

All lanes:

Western blot - Anti-Norbin antibody (ab224451) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat cell line) cell lysate

Predicted band size: 79 kDa

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Supplier Data

Western blot - Anti-Norbin antibody (AB224451)

All lanes:

Western blot - Anti-Norbin antibody (ab224451) at 1/100 dilution

All lanes:

human cerebral cortex tissue lysate

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NCDN aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Norbin also known as neurochondrin is a protein with a mass of approximately 80 kDa. It is expressed widely in the central nervous system with significant levels present in neurons. Norbin plays a mechanical role by interacting directly with various signaling proteins acting as an anchor within cell signaling frameworks. The interactions help regulate multiple cellular processes important for neuronal function and communication indicating Norbin's significance in neurophysiological activities.

Biological function summary

The actions of proteins within the cell often influence cellular signaling and communication. Norbin is a part of several protein complexes assisting in the regulation of signal transduction pathways particularly those involving G-protein-coupled receptors (GPCRs). These interactions highlight Norbin's role in maintaining synaptic function and neuronal excitability. Norbin also affects synaptic plasticity contributing to learning and memory processes by modulating neurotransmission efficiency.

Pathways

Various signaling processes within cells are interlinked and Norbin integrates into the calcium signaling and MAPK pathways. In these pathways Norbin interacts closely with proteins like mGluR5 (a type of metabotropic glutamate receptor) to mediate downstream effects on neuronal activity and development. This association impacts cellular processes by controlling cellular responses to environmental stimuli thereby affecting overall nervous system functionality.

Proteins and pathways often correlate with specific conditions. Norbin's involvement with neurophysiological pathways links it to neurological disorders such as schizophrenia and Alzheimer's disease. During disease states altered Norbin expression or dysregulation may impact its interaction with proteins like mGluR5 exacerbating symptoms or progression of these conditions. Research into Norbin's role offers potential therapeutic insights into these neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Probably involved in signal transduction in the nervous system, via increasing cell surface localization of GRM5/mGluR5 and positively regulating its signaling (PubMed : 33711248). Required for the spatial learning process. Acts as a negative regulator of Ca(2+)-calmodulin-dependent protein kinase 2 (CaMK2) phosphorylation. May play a role in modulating melanin-concentrating hormone-mediated functions via its interaction with MCHR1 that interferes with G protein-coupled signal transduction. May be involved in bone metabolism. May also be involved in neurite outgrowth (Probable).

See full target information NCDN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 39:877-887 PubMed37750664

2023

Selenomethionine suppresses head and neck squamous cell carcinoma progression through TopBP1/ATR and TCAB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Xiaodong Wei,Jiwu Li

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Norbin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Norbin antibody (AB224451)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norbin antibody (AB224451)

Western blot - Anti-Norbin antibody (AB224451)

Western blot - Anti-Norbin antibody (AB224451)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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