Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20]

7 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Negative control: no staining on rat pancreas.
  
  The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Western blot - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  The band beneath the target band (62 kDa) is  expected to be degradation products.
  
  In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 3 seconds

  All lanes: Western blot - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068) at 1/1000 dilution

  Lane 1: 293T whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a Capsid protein VP1 expression vector containing a his tag whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 62 kDa

  Exposure time: 3s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Negative control: no staining on human pancreas (PMID:19266182).
  
  The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Negative control: no staining on mouse pancreas.
  
  The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Immunohistochemical analysis of paraffin-embedded cells labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Capsid protein VP1 expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag.
  
  The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Flow Cytometry (Intracellular) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged Capsid protein VP1 overexpression construct cells labelling Norovirus Capsid protein VP1 with ab308068 at 1/5000 dilution (Right) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab308068 and co-stained with anti-Myc tag conjugated to Alexa Fluor® 647.

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  Indirect ELISA - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (ab308068)

  Indirect ELISA analysis of ab308068 at 0-1000 ng/ml.
  
  The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
  
  Antigen: Capsid protein VP1