Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human pancreas (PMID : 19266182). The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat pancreas. The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse pancreas. The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded cells labeling Norovirus Capsid protein VP1 with ab308068 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Capsid protein VP1 expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab308068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged Capsid protein VP1 overexpression construct cells labelling Norovirus Capsid protein VP1 with ab308068 at 1/5000 dilution (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left) A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab308068 and co-stained with anti-Myc tag conjugated to Alexa Fluor® 647.

• I-ELISA

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Indirect ELISA - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using ab308068, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab308068 at 0-1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution. Antigen : Capsid protein VP1

• WB

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Western blot - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] - BSA and Azide free (AB308069)

This data was developed using 308068, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST The band beneath the target band (62 kDa) is  expected to be degradation products. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-Norovirus Capsid protein VP1 antibody [EPR27186-20] (<a href='/en-us/products/primary-antibodies/norovirus-capsid-protein-vp1-antibody-epr27186-20-ab308068'>ab308068</a>) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Capsid protein VP1 expression vector containing a his tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 62 kDa

false

Exposure time: 3s