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AB320001

Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free

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Mouse Recombinant Monoclonal CAPSD antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Recombinant fragment - Norovirus samples.

View Alternative Names

VP1

3 Images
Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)
  • WB

Lab

Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)

This data was developed using ab256369, the same antibody clone in a different buffer formulation.

False colour image of Western blot : Anti-ORF2 antibody [SM59] (ab256369) staining at 1/1000 dilution, shown in green. In Western blot, ab256369 was shown to bind specifically to ORF2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution.

All lanes:

Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] (<a href='/en-us/products/primary-antibodies/norovirus-capsid-protein-vp1-antibody-sm59-ab256369'>ab256369</a>) at 1/1000 dilution

Lane 1:

Norovirus Group-1 Capsid Recombinant Protein cell lysate at 0.1 µg

Lane 2:

Norovirus Group-2 Capsid Recombinant Protein cell lysate at 0.1 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution

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Indirect ELISA - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)

This data was developed using ab256369, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab256369 at 1000-0 ng/ml. The Secondary antibody used was Goat Anti-Mouse IgG Fc (HRP) at 1 : 2000 dilution.

Antigen : Norovirus Group-1 Capsid Recombinant Protein, Norovirus Group-2 Capsid Recombinant Protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)
  • WB

Supplier Data

Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] - BSA and Azide free (AB320001)

This data was developed using ab256369, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 3% NFDM/TBST.

All lanes:

Western blot - Anti-Norovirus Capsid protein VP1 antibody [SM59] (<a href='/en-us/products/primary-antibodies/norovirus-capsid-protein-vp1-antibody-sm59-ab256369'>ab256369</a>) at 1/1000 dilution

All lanes:

Norovirus Group-1 Capsid Recombinant Protein at 100 ng

Secondary

All lanes:

Goat anti-Mouse IgG (IRDye®800CW) at 1/20000 dilution

Observed band size: 56 kDa

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  • Unconjugated

    Anti-Norovirus Capsid protein VP1 antibody [SM59]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SM59

Isotype

IgG1

Carrier free

Yes

Reacts with

Norovirus

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has been tested with recombinant Norovirus Capsid protein VP1 protein only.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Norovirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Norovirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab320001 is the carrier-free version of ab256369.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Norovirus Capsid protein VP1 also known as the Norwalk Capsid protein is a major structural component of noroviruses. The mass of this protein is approximately 58 kilodaltons. It is expressed within the viral particles forming the outer shell or capsid which plays an important role in protection and infectivity of the virus. VP1 is also the main target for capsid ELISA tests which are employed for virus detection.
Biological function summary

Norovirus Capsid protein VP1 assembles into icosahedral particles forming the structure that encases the viral RNA. It works as part of a complex with other molecular groups to facilitate the virus’s entry into host cells by binding to histo-blood group antigens found on epithelial cells of the digestive tract. This protein’s structure allows it to adapt and evade the host immune response which plays role in the virus's infectivity.

Pathways

Norovirus Capsid protein VP1 involves in pathways related to viral replication and immune evasion. Inside the host it participates in interactions that inhibit immune signaling pathways. These interactions often involve proteins like the Golgi-associated mechanics to promote efficient assembly and release of viral particles. VP1's interaction with cellular machinery ensures ongoing propagation of the virus.

The Norovirus Capsid protein VP1 directly connects to gastroenteritis a leading cause of acute viral gastroenteritis. It links to disease progression by aiding viral invasion and persistence in the gastrointestinal tract. The VP1's interactions with the immune system and histo-blood group antigens contribute to high transmissibility and variability of infection severity among different individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Norovirus Capsid protein VP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com